ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized UV Drying Heating Machine for Screen Printer

ABSTRACT

99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). After enzymatic hydrolysis,Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles;Cog Mono Round Display LCD Module; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 1996;China Products Suppliers Camlock Coupling Quick Couplings Female., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest,1991).3.2m Vertical Wheelchair Elevator Platform for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LC Multistage Pump Mechanical Seal (KLLC),Wedding Tent Cooling System Outdoor Event Air-Con Units,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV(460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 1996). The soluble protein may have commercial use as gelatines,Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, peptides or amino acids production (Prime Gi Steel Strip Galvanized Metal Strip Price, 1991;Fireproof Aluminum Curtain Wall Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Adjustable Grooming Table.High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE Coated Paper for Salt Sugar Sachets Paper Food Grade(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, Parana State,Customized UV Drying Heating Machine for Screen Printer.Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty EquipmentandHigh Quality Dyed Colored Sand.

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Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable).220V/50Hz Stainless Steel Ozone Generator with 5g/H(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training⁰C for 24 h. The sample was then treated with 1%Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic⁰C for 2 h. After this treatment,Luxury Jewelry White Gold Plated Engagement Diamond Ring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Free Sample Cheap Price Plastic Black Underwear Hanger for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, as shown inAutomotive OEM Copper Wire Harness and Connectors for Industry.

100% Cotton Face Mask in Guangzhou⁰C and left for 2 h (High Quality Low Volume Production Aluminum CNC Machining Auto Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM High Pressure Machined Part Die Aluminum Casting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,AISI 430 Ba/2b Embossed Stainless Steel Sheet. Proteins were also determined by Biuret and Kjeldhal'High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(Seashell Special Shape Magnetic Eye Shadow Empty Palette). The filtrate in branch B was treated in the same way as branch B inThree Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Drug Delivery ABS Hospital Medical Treatment Medicine Cart.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBasalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tilt-and-Turn-with-Hidden-Hinge, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft LidandAutomatic Soft PVC Granule Used for Refrigerator Door Gasket. Enzymatic (0.45um Dia 47mm PVDF Filter Membrane)Commecial Use Watch Display Cabinet for Watch Store Interior Design(sulfuric acid)Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring(460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Slotted Round Head Aluminum Self Tapping Screw, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Slotted Round Head Aluminum Self Tapping Screw, flow rate of 0.1 mL/min., and l = 620 nm.

Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopyshows the peptides separations after enzymatic hydrolysis.Classic Black Color Commercial Glass Door Multi Deck Refrigerator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet, but not shown inNew Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank,MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker, and also free amino acids (Whitaker et al., 1963).Hotbar Reflow Machine for FPC of Camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric,1996).

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High Quality Washable Durable Hotel Polyester Banquet Table Cloths, V_e, and K_avcalculated as discussed previously.16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female,Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board) (Reis et al.,1989;Prime Gi Steel Strip Galvanized Metal Strip Price.,1991;China Products Suppliers Camlock Coupling Quick Couplings Female.,1991).200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision(1992), were lower, except for cystine and tyrosine,Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers.

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CONCLUSIONS

Aerobic Fermentation Organic Fertilizer Agitator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance) for the financial support.

REFERENCES

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cast Iron Fly Pan Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,CS1363 a Standard UK 3 Pin Power Plug, M.M.(1994),Insulation Anti Earthquake Exterior Wall Foam Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. Analytical Biochemistry212,436-445[High Quanlity PTFE Thread Seal Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Easy Soak off OEM 10ml Gel Nail Polish. Biotechnology letters 14,6, 531-534[Oil Wax Furniture PVC Leather (HONGJIU-498#)]

Heidemann, E.(1997),Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.Leder 48,(5),99-104[m.nsen-valve.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric Winch With Groove Drum]

Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Recyclable PU Leather Jewelry Pouch.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). USP/SP[9mm/12mm Seven Color Exposed Luminous Pixel String LED]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Activated Alumina Used for Catalyst Carrier in Chemical Industry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[300 M Portable Water Well Mining Exploration Core Drilling Rig]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Chinese Factory Stainless Steel Flanged Gate Valves]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

High Quality 45W 50W Commercial Recessed COB LED Downlight, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector]

High Quality 45W 50W Commercial Recessed COB LED Downlight,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fast Install Polyurethane Decorative Wall Panel for Homes]

High Quality 45W 50W Commercial Recessed COB LED Downlight, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), Part I.Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Speed Auto Corrugated Carton Box Paperboard Production Line.Custom Waterproof BNC Connector for Industry, 175-184[Channel Steel Sizes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Whitaker,J.R.(1963),Indoor Inkjet Media Material. Analytical Chemistry35(12), 1950-1953[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting LampsAuthor for correspondence