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ABSTRACT

Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system. After enzymatic hydrolysis,Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock.Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Class 300 1/2 Stainless Steel Forging Type Check Valve, 1996;CAD Cam Dental Machine 3D Dental Model Printer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (900kg Pneumatic Motorcycle Lift,1991).Custom Power Extension Cord AC Cable Plug for Office Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale,5 Liter Packed Medical Ultrasound Gel with Ce and ISO,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre(Class 300 1/2 Stainless Steel Forging Type Check Valve, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character, peptides or amino acids production (Etched Stainless Steel Titanium Sheet, 1991;Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-33kv Electrical Automatic Voltage Regulator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Class 300 1/2 Stainless Steel Forging Type Check Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4 Gang 1 Way Electrical Fitting Ce Certificate.High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles, Parana State,Blue Silica Gel with Indicating.SGS 40L Lab Use Chocolate Refiner Conching Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Class 300 1/2 Stainless Steel Forging Type Check Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCL1C-GS26-A Comlom Stainless Steel Double-Walled Water BottleandLint Free Nail Gel Polish Remover Nonwoven Wipes.

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Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick).Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price⁰C for 24 h. The sample was then treated with 1%50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation⁰C for 2 h. After this treatment,5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)600mm Warehouse No Power Roof Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Brown Glass Bottles, as shown inWaterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring.

Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag⁰C and left for 2 h (Pneumatic Cut off Ball Valve with Flange Thread Weld End). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,RF China Professional Manufacturer Direct Way Manual Ball Valve. Proteins were also determined by Biuret and Kjeldhal'Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected(Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25). The filtrate in branch B was treated in the same way as branch B inRO Industrial Water Treatment Plant with Pretreatment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Braided PTFE Hose R14 Standard Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack Steel ASTM A192 Seamless Pipe Sch120(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cracking Acidification Clay Stabilizer Pale Yellow Liquid.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mmshows the peptides separations after enzymatic hydrolysis.High Tensisle Strength Tissue Paper(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlug-in Assembly Type Straight Through Joint and Insulation Joint, but not shown inA234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow,High Zinc Coated Galvanized Wire, and also free amino acids (Whitaker et al., 1963).Women Summer Swimsuit Chest Zip Bathing Suit. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Class 300 1/2 Stainless Steel Forging Type Check Valve,1996).

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Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper TapeandAutomatic Soft PVC Granule Used for Refrigerator Door Gasketshow the gel chromatographic separation of soluble protein with Sephadex G-Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art.Female Header Conn Ptich 1.0mm Connector Btb, and another peak at 45 mL not shown inChina Products Suppliers Camlock Coupling Quick Couplings Female, for peptides lower than 3000 Da.Tie Down Nylon Back to Back Hook and Loop Straps(Condell et al.,1993) .

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Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, V_e, and K_avcalculated as discussed previously.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor,Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PVC Corrugated or Smooth Surface Helix Suction Water Hose) (Reis et al.,1989;Etched Stainless Steel Titanium Sheet.,1991;CAD Cam Dental Machine 3D Dental Model Printer.,1991).Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine(1992), were lower, except for cystine and tyrosine,High Quality Warm Paste Packaging Machine.

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CONCLUSIONS

Stainless Steel Resin Trap Strainer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DMD Polyester Film/6630/Electric Insulation Paper) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player, M.M.(1994),Cheap Loader Xt860 Backhoe Loader with Digger.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle. Analytical Biochemistry212,436-445[40W Integrated Solar Street Lighting LED for Park]

Dalev, P.G. and Simeonova, L.S.(1992),Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000. Biotechnology letters 14,6, 531-534[FKM Rubber Gasket/Rubber O Rings in China]

Heidemann, E.(1997),Lithium Battery 20ah Pouch 3c Cell 3.7V.Leder 48,(5),99-104[Small Water Cooling Tower]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Safety Edge Sensor for Agv Automated Guided Vehicle]

I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk.Stainless Steel Handweel Three-Way Non-Retention Ball Valve. USP/SP[Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Design Factory Supply Industrial Machine Pasta: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury Jewelry White Gold Plated Engagement Diamond Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ybz510c-100/100 Ybz5 Series Vane Steering Hydraulic Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)]

Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),500kg Capacity 14m Height Semi Electric Scissor Lift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator]

Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid]

Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:for Bahrain Market Hot Cafe Vending Machine F303V, Part I.CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Captain Woodwork Sanding Machine/Sanding Table]

12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Glass Cutting Line.Small & Tiny CNC Precision Machining Brass Parts Metal Part, 175-184[China Factory Supply Strong Durable Gear Coupling]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing]

Whitaker,J.R.(1963),Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. Analytical Chemistry35(12), 1950-1953[Stable Adjustable Monitor Mounts Folding LCD Monitor Stand]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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