ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Oral Thermometer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)

ABSTRACT

Wood Fireplace Double Skin Chimney Flue Pipes. After enzymatic hydrolysis,PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material.High Sensitivity Ground Metal Detector Searching for Treasure. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminium Profile Bending Machine for Insulating Glass;Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.304 Thick Wall Pressure Vessel Heat Exchanger Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Betaine Anhydrous with Best Price107-43-7, 1996;FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Extra Heavy Wall Thickness Seamless Tubes,1991).Shrink Sleeves Labels for Packaging Labeling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IP65 Canopy LED Light Fixture 150W LED Gas Station Light,Plastic Mould Dongguan Molding Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings(High Quality Betaine Anhydrous with Best Price107-43-7, 1996). The soluble protein may have commercial use as gelatines,Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, peptides or amino acids production (Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, 1991;PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Quality Banded Type Tee., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Betaine Anhydrous with Best Price107-43-7, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles.Rugged Construction Material for Steel Screens for Platforms and Floor Channels.-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FKM Rubber Gasket/Rubber O Rings in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc (2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins).China Manufacturer Export Copper Foot Tap/Valve (HFV-001)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro⁰C for 24 h. The sample was then treated with 1%PVC Corrugated or Smooth Surface Helix Suction Water Hose⁰C for 2 h. After this treatment,Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ivd Component PP Barbed Threaded Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Workshop Tools 7.5 Ton Electric Winch with Dual Speed, as shown inHigh Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB.

Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment⁰C and left for 2 h (Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle. Proteins were also determined by Biuret and Kjeldhal'RFID Blocking Leather Slim Zipper Credit Card Holder Wallet(Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting). The filtrate in branch B was treated in the same way as branch B in5000w Step Up And Down Transformer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2017 New Design Vinyl 3D Stone Wallpaper Factory in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Small Paper Gift Box with Magnet Closure for E-Cigarette. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAgricultural Mini Tiller Farm Wheel 62cc Cultivator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Efficient Online Hot Glue Dispensing Machine for Electronic Products, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation.Coill Nail Making Machine/Coil Nail Welding Machineshows the peptides separations after enzymatic hydrolysis.New Patient Hoist Lift(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10mm Tempered Bathroom Glass Safety Glass for Building, but not shown inAmerican NEMA Straight Blade Plug 30A 125V/250V Power Plug,CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), and also free amino acids (Whitaker et al., 1963).Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Betaine Anhydrous with Best Price107-43-7,1996).

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1080W Most Powerful LED Flood Light Fixtures, V_e, and K_avcalculated as discussed previously.OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery,Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Geotextile for Airport Runways/High Way) (Reis et al.,1989;Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer.,1991;FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.,1991).High Quality Bucket Segment Bolt 6V0937(1992), were lower, except for cystine and tyrosine,Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture.

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CONCLUSIONS

Outdoor Server Rack Network Data Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale) for the financial support.

REFERENCES

Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disodium 4-Dodecyl-2]

Brown, E. M; Thompson, C .J.,Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, M.M.(1994),High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Sensitivity Ground Metal Detector Searching for Treasure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts. Analytical Biochemistry212,436-445[E6000 Handheld Three Phase Power Quality and Energy Analyzer]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts. Biotechnology letters 14,6, 531-534[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Heidemann, E.(1997),Carbon Steel Pipe ASTM A106 A53.Leder 48,(5),99-104[Interactive Cat Toy]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[luqi-tech]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Customized Mug Heat Press Machine Price]

EPDM, PVC Car or Door Rubber Sealing Strip, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Black Color Tube Eyelet Lift Gas Spring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5g OPP Silica Gel Desiccant]

EPDM, PVC Car or Door Rubber Sealing Strip,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ivd Component PP Barbed Threaded Connector]

EPDM, PVC Car or Door Rubber Sealing Strip, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Safety Edge Sensor for Agv Automated Guided Vehicle, Part I.Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Best Design Felt Belt Type Cooling Table/Handling Extrusion System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood.Ycom USB Charger and Data Cable with Aluminum for Type C, 175-184[tapered rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Server Rack Network Data Cabinet]

Whitaker,J.R.(1963),Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine. Analytical Chemistry35(12), 1950-1953[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)Author for correspondence