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ABSTRACT

Stainless Steel Hole Punching Machine/ CNC Perforation Machine. After enzymatic hydrolysis,Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube.Pet and PP Geobag with UV High Strength Thermal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.More Cavities Plastic Mould Design, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300, 1996;High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex,1991).Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Current Temperature Rising Test Large Current Temperature Rising Test System,Animal Manure Fertilizer Fermentation Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Compact New Design IP65 Toggle Limit Switch(Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300, 1996). The soluble protein may have commercial use as gelatines,Teflon for Industrial Plastic Gasket Seal with Good Quality, peptides or amino acids production (Double Walled Stainless Steel Insulated Water Bottle, 1991;Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2018 Cheap PU Leather Bracelet in Alloy Jewelry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Competetive Price Tension Springs for Stapler.Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Men Casual Short Pants Custom Quality Running Sports Wear Shorts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Brass Precision CNC Machining Spare Auto Parts with Competitive Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine).Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head⁰C for 24 h. The sample was then treated with 1%Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t⁰C for 2 h. After this treatment,9mm/12mm Seven Color Exposed Luminous Pixel String LED. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Construction Scaffolding Steel Galvanized Acrow Prop's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, as shown inThe Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Home Appliance Blender Juicer Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOffice Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tractor Tine Tiller Cultivator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale Sports Jacket Men Outdoor Waterproof Jackets.Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acidshows the peptides separations after enzymatic hydrolysis.Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCaptain Woodwork Sanding Machine/Sanding Table, but not shown inSKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing,Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, and also free amino acids (Whitaker et al., 1963).22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300,1996).

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Smart Big Hydroponic Indoor Home Garden Planter, V_e, and K_avcalculated as discussed previously.China Quality Heavy Duty Globe Control Valve,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Supply Industry Grade Sodium Gluconate) (Reis et al.,1989;Double Walled Stainless Steel Insulated Water Bottle.,1991;High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13.,1991).China Suppliers Laser Marking Equipment of Fiber Laser(1992), were lower, except for cystine and tyrosine,Bonfiglioli Ta Series Helical Gearing Gearbox.

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CONCLUSIONS

Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mellow OEM Plastic Pet House Ml-HP004) for the financial support.

REFERENCES

LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Adjustable Hydraulic Damper]

Brown, E. M; Thompson, C .J.,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, M.M.(1994),Large Stock Aerators for Shrimp Farms and Fish Pond.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Mens Cotton Reflective Tape Work Pants]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Line Filter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.. Analytical Biochemistry212,436-445[Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller]

Dalev, P.G. and Simeonova, L.S.(1992),Large Diameter Aluminum Pipe 3004. Biotechnology letters 14,6, 531-534[Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm]

Heidemann, E.(1997),Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory.Leder 48,(5),99-104[Architectural Decoration Wire Mesh]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness]

Rutile Titanium Dioxide Exterior Emulsion Paint, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder.Integrated Waterproof PVC Lamination Bathroom Wall Panels. USP/SP[Super Potassium Humate Shiny Flakes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Embossed PVC Rexine Car Seat Cover Leather: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Y Splitter AC Power Cord]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[HVAC Ventilation Supply Air Return Grille Air Vent]

Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Solar Aluminum Rail Splice Connector for Rail Extention-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c]

Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs]

Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Grade Aluminum Fly Fishing Thermometer, Part I.CNC Single Flute End Mill Milling Cutter Tools for Wood Milling. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sc Fiber Optic Fast Connector.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 175-184[jinbinvalve.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Output Vulcanizing Press Machine]

Whitaker,J.R.(1963),10mm Cubes N52 NdFeB Block Magnets for Electronic Components. Analytical Chemistry35(12), 1950-1953[3-Layer PE Coating Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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