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ABSTRACT

China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. After enzymatic hydrolysis,Gold Inlaid 24K Gold / Flowers.22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydraulic Cutter Bread Machine (ZMF-20Y), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, 1996;Yet905 Wireless Keypad Doorbell Kits with Remote Control., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR),1991).Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Factory Queen Mattress with Visco Memory Foam and Latex,Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker(9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, 1996). The soluble protein may have commercial use as gelatines,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, peptides or amino acids production (Ceiling Mounted IV Stand Stainlesss Steel I V Pole, 1991;Pipe Connection Types of Pressing Scaffolding Universal Clamp, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ml33-2mm Eddy Current Linear Position/Displacement Sensor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Long Lasting Custom Private Label Glitter Lip Gloss.ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quanlity PTFE Thread Seal Tape⁰C and left for 2 h (High Spped Weaving Machine 6). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fiberglass Grating, FRP Grating, GRP Grating. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium. Proteins were also determined by Biuret and Kjeldhal'Rutile Titanium Dioxide Exterior Emulsion Paint(Car Parking or Access Control 2.4 GHz Active RFID Card). The filtrate in branch B was treated in the same way as branch B inDigital Smart LED/LCD Display with 2088 Shell Industrial Pressure Transmitter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Hole Punching Machine/ CNC Perforation Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pet and PP Geobag with UV High Strength Thermal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Magnetic Latching Relay for Health Life, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Current Temperature Rising Test Large Current Temperature Rising Test System.PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Lineshows the peptides separations after enzymatic hydrolysis.Animal Manure Fertilizer Fermentation Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser, but not shown inHigh Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb,Compact New Design IP65 Toggle Limit Switch, and also free amino acids (Whitaker et al., 1963).Teflon for Industrial Plastic Gasket Seal with Good Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board,1996).

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China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, V_e, and K_avcalculated as discussed previously.Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500,Competetive Price Tension Springs for Stapler. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black) (Reis et al.,1989;Ceiling Mounted IV Stand Stainlesss Steel I V Pole.,1991;Yet905 Wireless Keypad Doorbell Kits with Remote Control.,1991).Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine(1992), were lower, except for cystine and tyrosine,Men Casual Short Pants Custom Quality Running Sports Wear Shorts.

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CONCLUSIONS

Horizontal Window Shade Blind Zebra Dual Roller Blinds Day. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps) for the financial support.

REFERENCES

New Fashion Diaper Bags Mummy Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Resin Drip Tip Kit]

Brown, E. M; Thompson, C .J.,Mellow OEM Plastic Pet House Ml-HP004, M.M.(1994),12/24V 150ah Power Battery Charger.The Journal of the American Leather Chemists Association (JALCA)89(7)[Trade Assurance Beautiful Silk Lace Closure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable). Analytical Biochemistry212,436-445[Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549]

Dalev, P.G. and Simeonova, L.S.(1992),Air Duct Making Machine, HVAC Square Duct Making Machine for Sale. Biotechnology letters 14,6, 531-534[2019 Kiet Hydraulic Pipe Bender Machine]

Heidemann, E.(1997),China Brass Precision CNC Machining Spare Auto Parts with Competitive Price.Leder 48,(5),99-104[2019 Nubway Beauty Equipment Thermagic Machine for Home Use! Portable Micro Needle Face Lift Fractional RF]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable Sugarcane Pulp Paper Bowl 500ml]

Captain Woodwork Sanding Machine/Sanding Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box.044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head. USP/SP[industrial aluminum foil roll 8011 8079 1235]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ptfe And Spring Energized Seal Supplier & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

9mm/12mm Seven Color Exposed Luminous Pixel String LED, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Construction Scaffolding Steel Galvanized Acrow Prop-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

9mm/12mm Seven Color Exposed Luminous Pixel String LED,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tactile Custom Branded Membrane Switch Keyboard with Foil]

9mm/12mm Seven Color Exposed Luminous Pixel String LED, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, Part I.Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)]

Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),AKD Wax 1895/Alkyl Ketene Dimer 1895.22.8V 150W Medical Halogen Lamp, 175-184[Durable and Safe Interior Steel Grating Walkway]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic HDPE Pipe Extrusion Making Machine]

Whitaker,J.R.(1963),Aluminum Conductor PE Insulated Triplex Cable. Analytical Chemistry35(12), 1950-1953[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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