ARTICLES

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ABSTRACT

Professtional Suppliers Yutong Golden Dragon Bus Spare Parts. After enzymatic hydrolysis,Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate.Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxury Jewelry White Gold Plated Engagement Diamond Ring, 1996;Free Sample Cheap Price Plastic Black Underwear Hanger for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay,1991).100% Cotton Face Mask in Guangzhou. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM High Pressure Machined Part Die Aluminum Casting,AISI 430 Ba/2b Embossed Stainless Steel Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(Luxury Jewelry White Gold Plated Engagement Diamond Ring, 1996). The soluble protein may have commercial use as gelatines,Drug Delivery ABS Hospital Medical Treatment Medicine Cart, peptides or amino acids production (YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, 1991;Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Colorful Electric English Funny Music Story Module Printing Book., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxury Jewelry White Gold Plated Engagement Diamond Ring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tilt-and-Turn-with-Hidden-Hinge.Automatic Soft PVC Granule Used for Refrigerator Door Gasket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.45um Dia 47mm PVDF Filter Membrane(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hotbar Reflow Machine for FPC of Camera(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c).Full Viton Coated Half Shaft Wafer Butterfly Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Claus Process Catalyst 4-6 mm⁰C for 24 h. The sample was then treated with 1%4 Different Ball Silicone Ice Cube Maker⁰C for 2 h. After this treatment,High Quality Washable Durable Hotel Polyester Banquet Table Cloths. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, as shown inYuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings.

200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision⁰C and left for 2 h (Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aerobic Fermentation Organic Fertilizer Agitator. Proteins were also determined by Biuret and Kjeldhal'Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment(Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools). The filtrate in branch B was treated in the same way as branch B inGlass Display. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CS1363 a Standard UK 3 Pin Power Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Insulation Anti Earthquake Exterior Wall Foam Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avZhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Recyclable PU Leather Jewelry Pouch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Slotted Round Head Aluminum Self Tapping Screw) for the financial support.

REFERENCES

Small Poultry Pig Feed Farm Steel Silo for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bamboo Fitted Sheet]

Brown, E. M; Thompson, C .J.,Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, M.M.(1994),P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Slide Switch - DC Power Jack RoHS]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Made in China 12 Inch Aluminum Flexible Duct Pipe. Analytical Biochemistry212,436-445[Tractor Tine Tiller Cultivator]

Dalev, P.G. and Simeonova, L.S.(1992),18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring. Biotechnology letters 14,6, 531-534[Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides]

Heidemann, E.(1997),Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food.Leder 48,(5),99-104[Hot Melt Dispensing Units Glue Dispenser for Bags (LBD-RT1016)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring]

Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Safe Delivery Effective Sarms Mk 677 for Bodybuilding.12# X 14# Electric Galvanized Double Strands Barbed Wire. USP/SP[Car Trunk Organizer Car Storage Organizer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer CNC High Precision Machining Metal Parts Customized for Auto]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Service Machining Milling Turning Service Custom CNC Aluminum Case: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Capacity Automatic Cup Filling Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ISO SGS Certified Cordyceps Sinensis Tablet/Cordyceps Polysaccharide Tablet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China High Efficiency Automatic Glue Dispensing Machine with Factory Price]

Wholesale Mini School Backpack for Girls, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning]

Wholesale Mini School Backpack for Girls,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont]

Wholesale Mini School Backpack for Girls, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Super Potassium Humate Shiny Flakes, Part I.4K Giant Whiteboard Interactive Panel for University Whiteboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring]

Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric Massage Bed Pedicure Chair with Double Dust Collector.750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, 175-184[P4 Indoor LED Signs for Business for Stage Concert Glass Window See Through Transparent LED Signs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Aluminum Carpet Topping Stage Platform]

Whitaker,J.R.(1963),Adjustable Solar Mounting Kits (GD1059). Analytical Chemistry35(12), 1950-1953[Professtional Suppliers Yutong Golden Dragon Bus Spare Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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