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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Foteck SSR 40da Solid State Power SSR Relay, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2015 New Square Folding Ajustable Wall LED Bathroom Mirror, 1996;Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (13.8cm Reed Diffuser Clear Glass Bottle for Aroma,1991).Metal Cutting Machine CNC Plasma Cutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Suction Connecting Tube with Yankauer Suction Handle,Surface Mounted Wall Light for Home Decoration Waterproof,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant(2015 New Square Folding Ajustable Wall LED Bathroom Mirror, 1996). The soluble protein may have commercial use as gelatines,Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, peptides or amino acids production (Right Angle Worm Gearbox for Food and Beverage and Packaging Machines, 1991;Manufacturer High Strength Carbon Steel Heavy Hex Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Seamless Coil Tube ASTM A249/A269.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2015 New Square Folding Ajustable Wall LED Bathroom Mirror, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock.Hardware Rigging Forged Red Weldless Pear Shaped Link-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Water Pipe Insulation Steam(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Manufacturer Export Copper Foot Tap/Valve (HFV-001)).Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%45ton Proof Load Testing Water Bag for Life Boat⁰C for 24 h. The sample was then treated with 1%Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision⁰C for 2 h. After this treatment,Double Coil Control Solenoid Valve of Butterfly Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Frequency Online UPS W/3W-20K's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Solar Collector Panel for Pool Heater, as shown inSmart Big Hydroponic Indoor Home Garden Planter.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avA19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Home Design Solid Wood Double Adult Bed Bedroom Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade Beverage Clear PVC Tubing Hose) for the financial support.

REFERENCES

6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.fessiecookware.com]

Brown, E. M; Thompson, C .J.,Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification, M.M.(1994),Programmable Salt Spray Test Chamber for Corrosion Testing/Test.The Journal of the American Leather Chemists Association (JALCA)89(7)[5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%. Analytical Biochemistry212,436-445[Aluminum Sheet Metal Case Parts for Industrial Computer]

Dalev, P.G. and Simeonova, L.S.(1992),FKM Bearing Oil Seal /Customized. Biotechnology letters 14,6, 531-534[Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wall Mounting Metal Control Box Control Desk]

Nice Quality Coloring Printing Children Board Book Printing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile.Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce. USP/SP[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Max Twintier Rb441t Using Wire Coils Tw1061t: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Aluminum Brass Steel Custom Stamping Parts High Quality Low Price Small Batch Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Etched Stainless Steel Titanium Sheet]

Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturer Supply Durable Grey Color Non-Slip EVA Mat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Turned Aluminum Anodized Auto Part]

Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ml33-2mm Eddy Current Linear Position/Displacement Sensor]

Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Feed Storage OEM Grain Storage Silo 1cbm to 200cbm, Part I.Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

Captain Woodwork Sanding Machine/Sanding Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling.Atg39 100W LED Flood Light Wall Light Wall Washer, 175-184[Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Whitaker,J.R.(1963),China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. Analytical Chemistry35(12), 1950-1953[Custom Waterproof BNC Connector for Industry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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