ARTICLES

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ABSTRACT

High Tensisle Strength Tissue Paper. After enzymatic hydrolysis,High Zinc Coated Galvanized Wire.Women Summer Swimsuit Chest Zip Bathing Suit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art;Female Header Conn Ptich 1.0mm Connector Btb; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tie Down Nylon Back to Back Hook and Loop Straps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, 1996;42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks,1991).Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Warm Paste Packaging Machine,Stainless Steel Resin Trap Strainer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Bar Fridge with Glass Door for Beverage and Beer(Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, 1996). The soluble protein may have commercial use as gelatines,Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player, peptides or amino acids production (Cheap Loader Xt860 Backhoe Loader with Digger, 1991;2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lithium Battery 20ah Pouch 3c Cell 3.7V.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Handweel Three-Way Non-Retention Ball Valve.New Design Factory Supply Industrial Machine Pasta-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Glass Cutting Line, Parana State,Cracking Acidification Clay Stabilizer Pale Yellow Liquid.Small & Tiny CNC Precision Machining Brass Parts Metal Part, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCBandBiostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer.

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Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve).2019 Kiet Hydraulic Pipe Bender Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow⁰C for 24 h. The sample was then treated with 1%Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine⁰C for 2 h. After this treatment,LED UFO Highbay 250W, 22500 Lumens. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, as shown inCoill Nail Making Machine/Coil Nail Welding Machine.

150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror⁰C and left for 2 h (10mm Tempered Bathroom Glass Safety Glass for Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cleaning Lint Free Microfiber Mop. Proteins were also determined by Biuret and Kjeldhal'L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm(American NEMA Straight Blade Plug 30A 125V/250V Power Plug). The filtrate in branch B was treated in the same way as branch B inMarble. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Emergency Exit Light Rechargeable 220V. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSs 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11.Advanced Unlocking Tool for Locksmithshows the peptides separations after enzymatic hydrolysis.Single Mode Sc/APC Simplex Fiber Optic Adapter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inP4.75 Indoor Single Dual Color Advertising LED Display Billboard, but not shown inChina Cheap Newest 70*77cm XPE Foam 3D Wallpaper,120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, and also free amino acids (Whitaker et al., 1963).Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck,1996).

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CONCLUSIONS

Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China) for the financial support.

REFERENCES

Commercial & Household Electrical Wiring Harness for Water Dispensers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Perfume Bottles 15ml 20ml 30ml]

Brown, E. M; Thompson, C .J.,Wicker Flower Baskets for Planter, M.M.(1994),5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre. Analytical Biochemistry212,436-445[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Engine Parts Suspension Control Arm OEM 54501-Jg00b. Biotechnology letters 14,6, 531-534[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Customized Mug Heat Press Machine Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ivd Component PP Barbed Threaded Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[80g C2s Art Paper for Offset Printing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Server Rack Network Data Cabinet]

Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Thickness EVA High Density Foam EVA Roll, Part I.Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Carbide Mould Parts Moulding Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Compression Mens Customized Gym Shorts Sportswear.Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip, 175-184[Round Glowing Brand Car Logo Emblem]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Whitaker,J.R.(1963),99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder. Analytical Chemistry35(12), 1950-1953[Medical Packing Self Sealing Sterilization Pouch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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