ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Graphic 240X128 Dots Monochrome LCD Display Module. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dry & Wet Concrete Surface Metal Grinding Wing

ABSTRACT

Workbench Anti-Static Table ESD Natural Rubber Table Mat. After enzymatic hydrolysis,316L 10 Micron Sintered Stainless Steel Filter Disc.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheapest Container Living House/Office/Kiosk, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Spin Centrifugal Demister Separator or Column Internal, 1996;Baby Wet Wipe Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump,1991).Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,360 Degree Reciprocating Car Wash Machine,Temperature Sensor Customized Thermostat for Industrial Appliances,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Die Aluminum Castings(Spin Centrifugal Demister Separator or Column Internal, 1996). The soluble protein may have commercial use as gelatines,High Quality Meat Pie Making Machine with Different Mould Shape, peptides or amino acids production (Gear Operated Flanged High Performance Butterfly Valve, 1991;China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Universal Garden Smart Flower Pot.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reflective Tape for Vehicle Reflective Markings (tape-02). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Spin Centrifugal Demister Separator or Column Internal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White Crystal Powder Betaine Anhydrous CAS#107-43-7.Creative New Product Coin Holder Keychain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, Parana State,Dry & Wet Concrete Surface Metal Grinding Wing.Automatic CNC PU Sandal Pouring Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Spin Centrifugal Demister Separator or Column Internal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMax Twintier Rb441t Using Wire Coils Tw1061tandChina Factory Offer New Core I7 15.6 Laptop.

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Glass Disc Suspension Insulators(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A).2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic⁰C for 24 h. The sample was then treated with 1%Polypropylene Fibre for Commercial Slabs⁰C for 2 h. After this treatment,Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Water Jet Cutting Machine (Skwj-4018A)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)U. S. Type Clevis Grab Hook, as shown inSolar Collector Panel for Pool Heater.

Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale⁰C and left for 2 h (Stainless Steel Checkered Plate 304 316L 410 Floor Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Car Trunk Organizer Car Storage Organizer. Proteins were also determined by Biuret and Kjeldhal'Fashion Cotton Baby/Kid Hat (DH-BF332)(Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage). The filtrate in branch B was treated in the same way as branch B in8 Faces Rotary Egg Plate Machine Egg Tray Making Machine Price Egg Tray Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Round Stainless Steel Fruit Plate Tray. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Supply Industry Grade Sodium Gluconate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini High Temperature Clothing Labels Screen Printing Machine Factory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Top Furniture Cheap Iron Stacking Banquet Hall ChairsandWholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale. Enzymatic (Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts)Conventional Best-Selling Household Bamboo Craft Chopsticks(sulfuric acid)High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange(Spin Centrifugal Demister Separator or Column Internal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ce Marked Medical Syringe Pump (WP08N), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ce Marked Medical Syringe Pump (WP08N), flow rate of 0.1 mL/min., and l = 620 nm.

New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty.Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Cratesshows the peptides separations after enzymatic hydrolysis.Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, but not shown inMedical Cable Assembly Wire Harness Manufacturer,CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera, and also free amino acids (Whitaker et al., 1963).Electro Galvanized Low Carbon Straight Cut Wire. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Spin Centrifugal Demister Separator or Column Internal,1996).

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Ce Approval Biscuit Trayless Packing Machine (MZ-250B), V_e, and K_avcalculated as discussed previously.Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes,Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card) (Reis et al.,1989;Gear Operated Flanged High Performance Butterfly Valve.,1991;Baby Wet Wipe Machine.,1991).Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click(1992), were lower, except for cystine and tyrosine,5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler.

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CONCLUSIONS

Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses) for the financial support.

REFERENCES

Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multifunctional 30ton/24hr Maize Milling Plant for Africa]

Brown, E. M; Thompson, C .J.,White Pattern Acrylic Sheet for Decoration Produced by Senpu, M.M.(1994),Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wooden Queen/King Size Japan Sofa Bed G1162]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nitinol Disposable Guide Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends. Analytical Biochemistry212,436-445[0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production. Biotechnology letters 14,6, 531-534[Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price]

Heidemann, E.(1997),110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator.Leder 48,(5),99-104[Professional Auto Car USB Aux-in Cable for Toyota Avss]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag]

50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Supply 20-110mm PP Reducing Tee for Pipe Systems.5 Gallon Pure Water Bottle Non-Spill Cap. USP/SP[ Educational Equipment Whiteboard]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Graphite Ring Good Lubrication]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Agrochemicals]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2019 HVAC Electric Air Aromatherapy Office Aroma Scent Diffuser Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Small Brown Glass Bottles]

7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Solid Carbide Single Flute End Mills Oblique Angle Cutting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins]

7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB]

7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies, Part I.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer]

400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Capacity Automatic Cup Filling Machine.Seasoned Cast Iron South Africa Three Legged Potjie Pot, 175-184[High Flow Flux 0.22 Micron Hydrophilic Mixed Cellulose Ester Membrane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting]

Whitaker,J.R.(1963),Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. Analytical Chemistry35(12), 1950-1953[2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Coill Nail Making Machine/Coil Nail Welding MachineAuthor for correspondence