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ABSTRACT

New Style Custom Logo Makeup Multi Colored Eyeshadow Palette. After enzymatic hydrolysis,Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending.20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu;High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Daytai 1550nm External Modulation Optical Transmitter 1550 -50km, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, 1996;Hot Promotion Indian Market Underwear Adhesive Glue., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit,1991).Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Industrial Wheeled Wooden Shoe Display Fixtures for Shop,Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment(PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, 1996). The soluble protein may have commercial use as gelatines,Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light, peptides or amino acids production (High Quality Aluminum Carpet Topping Stage Platform, 1991;BY60100C type metal shaping machine/hydraulic shaper machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Spplier 70W Solar Street Light with Camera Monitoring System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard.Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avForged Steel Pressure Sealed Bonnet Globe Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manual Double Bridge Glass Cutting Table Machine (JFQG-2620), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. Design Engraved Logo Zipper Pullershows the peptides separations after enzymatic hydrolysis.Ck6150 High Precision Metal Machining Horizontal CNC Lathe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Brass Precision CNC Machining Spare Auto Parts with Competitive Price, but not shown inBlack Steel ASTM A192 Seamless Pipe Sch120,Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, and also free amino acids (Whitaker et al., 1963).Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PE100 Plasitc Pipe for Gas Water Chemcial Sewage System,1996).

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PVC Wood Grain Decorative Sheet, V_e, and K_avcalculated as discussed previously.Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer,LED UV Portable Toothbrush Sterilizer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Carbon Steel Equal Tee for Connection of Pipe) (Reis et al.,1989;High Quality Aluminum Carpet Topping Stage Platform.,1991;Hot Promotion Indian Market Underwear Adhesive Glue.,1991).Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding(1992), were lower, except for cystine and tyrosine,American NEMA Straight Blade Plug 30A 125V/250V Power Plug.

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CONCLUSIONS

10in 1500lb Pressure Seal Forged Steel Swing Check Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Masturbators Adult Sex Toys Japanese Love Doll) for the financial support.

REFERENCES

Custom Printed/Embossed Black Earring Packaging Cards, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Commercial Water Softener System]

Brown, E. M; Thompson, C .J.,Mug Printing Machine Heat Press Promotion Brazil, M.M.(1994),Automotive OEM Copper Wire Harness and Connectors for Industry.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Max Twintier Rb441t Using Wire Coils Tw1061t]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg. Analytical Biochemistry212,436-445[China Factory Offer New Core I7 15.6 Laptop]

Dalev, P.G. and Simeonova, L.S.(1992),En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692). Biotechnology letters 14,6, 531-534[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Doypack Machine/Doypack Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Furniture Cheap Iron Stacking Banquet Hall Chairs]

China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates]

China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370]

China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, Part I.Best Price Aluminium Sulphate for Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Cable Assembly Wire Harness Manufacturer]

Hot Sale Loading Truck with Cummins Engine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets.50kg Soya Bean Seed PP Woven Bags Sacks, 175-184[Light Grey Carpet Tiles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASTM A335 Seamless Steel Pipe by Grade P91]

Whitaker,J.R.(1963),Iron Wire Dry Type Straight Line Wire Drawing Machine. Analytical Chemistry35(12), 1950-1953[Screw Press Dewatering Machine for Handling The Reject From Paper Mill]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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