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ABSTRACT

Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. After enzymatic hydrolysis,High Quanlity Colorful Aluminum Spacer/Standoff.5g OPP Silica Gel Desiccant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Winch With Groove Drum;Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Truck Air Dryer cartridge 14bar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, 1996;3mm White Waterproof PVC Foam Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Educational Equipment Smart Multi Touch Interactive White Board for School,1991).Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Women Party Jewelry 45cm Sequins Choker,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe(Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, 1996). The soluble protein may have commercial use as gelatines,High Freqency PCB with Blind Via, peptides or amino acids production (Strong Aluminum Armrest Reclining Hydraulic Barber Chair, 1991;Factory SMD3528 60LEDs/M Flexible LED Strip, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot-Press Small Continuous Diamond Saw Blade Cutting Disc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Comfort Furniture Natural Spring Memory Foam Mattress.Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4.2A Type a Type C Pd QC3.0 USB Charger(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle).Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag⁰C for 24 h. The sample was then treated with 1%Customized UV Drying Heating Machine for Screen Printer⁰C for 2 h. After this treatment,99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, as shown inHigh Zinc Coated Galvanized Wire.

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles⁰C and left for 2 h (50kg Soya Bean Seed PP Woven Bags Sacks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cog Mono Round Display LCD Module. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. Proteins were also determined by Biuret and Kjeldhal'460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric(Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)). The filtrate in branch B was treated in the same way as branch B inUltrasonic (Sound). The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Products Suppliers Camlock Coupling Quick Couplings Female.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3.2m Vertical Wheelchair Elevator Platform for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wedding Tent Cooling System Outdoor Event Air-Con Units⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Adjustable Grooming Table.KZ-19/16/13 Pneumatic Combination Steel Binding Machineshows the peptides separations after enzymatic hydrolysis.High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, but not shown inBlue Silica Gel with Indicating,PE Coated Paper for Salt Sugar Sachets Paper Food Grade, and also free amino acids (Whitaker et al., 1963).Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors,1996).

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CONCLUSIONS

Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant) for the financial support.

REFERENCES

Luxury Jewelry White Gold Plated Engagement Diamond Ring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Curving Roof Machinery]

Brown, E. M; Thompson, C .J.,Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, M.M.(1994),13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Spindle Micro CNC Lathe Ck0640]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FKM Bearing Oil Seal /Customized]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% Cotton Face Mask in Guangzhou. Analytical Biochemistry212,436-445[044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head]

Dalev, P.G. and Simeonova, L.S.(1992),OEM High Pressure Machined Part Die Aluminum Casting. Biotechnology letters 14,6, 531-534[Vacuum Type Single Facer Corrugator]

Heidemann, E.(1997),AISI 430 Ba/2b Embossed Stainless Steel Sheet.Leder 48,(5),99-104[Packaging Paper Protector Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Handweel Three-Way Non-Retention Ball Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hardware Rigging Forged Red Weldless Pear Shaped Link]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hospital Diagnostic Equipment Portable Laptop B/W Ultrasound Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Colorful Electric English Funny Music Story Module Printing Book-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Explosion Proof Rechargeable LED Searchlight]

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, Part I.IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Garbage Bag Making Machine Price Trash Bag Sealing Machine]

Tilt-and-Turn-with-Hidden-Hinge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Soft PVC Granule Used for Refrigerator Door Gasket.0.45um Dia 47mm PVDF Filter Membrane, 175-184[www.nbwallplus.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Whitaker,J.R.(1963),Commecial Use Watch Display Cabinet for Watch Store Interior Design. Analytical Chemistry35(12), 1950-1953[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor TruckAuthor for correspondence