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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, 1996;High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet,1991).Tent Windows Use China Made PVC Transparent Clear Mesh Tarp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,13 Oz Flex Banner PVC Frontlit Banner Material,Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables(Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, 1996). The soluble protein may have commercial use as gelatines,Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, peptides or amino acids production (Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V, 1991;10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Different Size of Ring NdFeB Magnet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Investment Precision Casting for Valve Body with Machining.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Unique Best Quality Copper Home Alcohol Distiller for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories.Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Drive Travel Car Inflatable Bed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), Parana State,Dust Hood Fume Extraction Systems for Robotic Laser Cutting.Auto Spare Parts Ignition Coil 22448-2y001, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSafety Traffic Rubber Roof Support Feet Productsand300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.

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3D Good Quality Laser Engraving Machine for Souvenirs Shop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Reverse Flow Configuration Axial Fan).Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording⁰C for 24 h. The sample was then treated with 1%Reverse Flow Configuration Axial Fan⁰C for 2 h. After this treatment,Handcraft Wood Jewelry Storage Box with Lock and Key. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Single Spindle Micro CNC Lathe Ck0640, as shown inCustomized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet.

Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring⁰C and left for 2 h (Good Quality Banded Type Tee). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pipe Thermostat with Sensor Ce Certificate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line. Proteins were also determined by Biuret and Kjeldhal'Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring(Natural OEM Private Label Customized Skin Care Face Serum). The filtrate in branch B was treated in the same way as branch B inMono-Dicalcium Phosphate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nitinol Disposable Guide Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Breathable Surgical Paper Tape/ Micropore Medical Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (TPU TPR Rubber Band Plastic Extruder Machine) for the financial support.

REFERENCES

GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Mini LED Flood Light SMD Flood Light 150W]

Brown, E. M; Thompson, C .J.,Disposable Clear Take Away Plastic Salad Bowl Forming Machine, M.M.(1994),Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control.The Journal of the American Leather Chemists Association (JALCA)89(7)[Programmable Salt Spray Test Chamber for Corrosion Testing/Test]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP. Analytical Biochemistry212,436-445[Laser Cutting Machine for Sale for Die Shops From Yitai Die Making]

Dalev, P.G. and Simeonova, L.S.(1992),Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. Biotechnology letters 14,6, 531-534[Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring]

Heidemann, E.(1997),Carbon Stee Semless Pipe (ASTM A106 Gr B).Leder 48,(5),99-104[0410 axial inductor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Expanded Mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Selling House Floor Cleaning 360 Spin Mop: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Pressure Washer Plunger Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Advanced Unlocking Tool for Locksmith-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Watermark Approval Brass Press Equal Fitting Male Tee]

Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medical Suction Connecting Tube with Yankauer Suction Handle]

Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts, Part I.Fashion Design Sleeveless Fitness Wear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price Aluminium Sulphate for Water Treatment]

Whitaker,J.R.(1963),Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. Analytical Chemistry35(12), 1950-1953[Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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