ARTICLES

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ABSTRACT

Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042). After enzymatic hydrolysis,Replica Car Wheels with 20inch for BMW Car.SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cooling Tower Replaced Fill Media;A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Expanded Mesh, 1996;Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lf + Hf Contactless Smart Hybrid Card,1991).Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Fairy Star Light for All Kinds of Decoration Skylike,Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Linear Vibrator Screener, for Granule and Powder Selection and Classification(Stainless Steel Expanded Mesh, 1996). The soluble protein may have commercial use as gelatines,Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, peptides or amino acids production (Children Hardcover Book Printing Services with Free Printing File Checking, 1991;One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Global Brands 10 Year Cheaper Hook & Loop Tape Male Side., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Expanded Mesh, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Large Size Stone Aluminum Honeycomb Panel for Facade(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Efficiency Air Cooled Air Source Water Chiller Heat Pump, Parana State,19m Mobile LED Display Billboard for Advertising Screens.Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Expanded Mesh, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFull Color Print Glossy Laminated PP Woven Shopping Gift BagandFactory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks.

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12oz 16oz Glass Mason Jar with Handle and Straw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Precision Tooling Grinding Machine Part).Mini Freeze Dryer for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber⁰C for 24 h. The sample was then treated with 1%Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure⁰C for 2 h. After this treatment,CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality and Efficient Combustion UHP Graphite Electrode's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Graphite Ring Good Lubrication, as shown inVarious Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Double Cereal Airtight Food Dispenser. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDisposable Food Packaging Paper Soup Bowl with Lids(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stamping Fine Blanking Sheet Metal Fabrication Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Trade Assurance Beautiful Silk Lace Closure, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fully Automatic Digital Pid Temperature Controller, V_e, and K_avcalculated as discussed previously.High Quality Anti-Corrosion Fiberglass Sheet and Roll,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Disposable Skin Stapler (CSPF-35W)) (Reis et al.,1989;Children Hardcover Book Printing Services with Free Printing File Checking.,1991;Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2.,1991).Full-Automatic Folding Napkin Paper Machine(1992), were lower, except for cystine and tyrosine,Customized Frame Mount Highway Pegs Enclosure Parts.

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CONCLUSIONS

Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Making Machine for EVA Soft Hose) for the financial support.

REFERENCES

10mm Tempered Bathroom Glass Safety Glass for Building, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Pipe Y Fitting]

Brown, E. M; Thompson, C .J.,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, M.M.(1994),Cattle Feed Factory Used Dust Collector and Filter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Graphic 240X128 Dots Monochrome LCD Display Module]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Power Extension Cord AC Cable Plug for Office Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. Analytical Biochemistry212,436-445[Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts]

Dalev, P.G. and Simeonova, L.S.(1992),Group 152cm Picnic Folding in Half Table. Biotechnology letters 14,6, 531-534[2018 New Style High Quality Enamel Bowl with Lid]

Heidemann, E.(1997),Tactile Custom Branded Membrane Switch Keyboard with Foil.Leder 48,(5),99-104[3003 Aluminium Coil 0.5mm Thickness for Transformer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. USP/SP[2018 Cheap PU Leather Bracelet in Alloy Jewelry]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rubber Gasket for Radiator Plastic Tanks Rubber Glazing Gaskets Pump Rubber Gasket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Spin Centrifugal Demister Separator or Column Internal]

Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Australia Two Pins Power Cord with SAA Certification-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Kellogg′s Corn Flakes Cereals Snacks Food Making Machine]

Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tongkat Ali Root Extract 200 1]

Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, Part I.Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml]

Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, 175-184[Superior Quality High Impact Strength UHMWPE Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud]

Whitaker,J.R.(1963),Pickled Cucumber Production Line/Making Machine. Analytical Chemistry35(12), 1950-1953[Barricade Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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