ARTICLES

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ABSTRACT

125mm Wet Diamond Pad for Polishing Marble. After enzymatic hydrolysis,Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter.40W Integrated Solar Street Lighting LED for Park. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Checkered Plate 304 316L 410 Floor Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, 1996;Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection,1991).Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports,Sport Anti Vibration Hiking Safety Shoes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial(Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, 1996). The soluble protein may have commercial use as gelatines,Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, peptides or amino acids production (24W 400W Rainproof Switch Power Adapter for LED Light, 1991;Industrial Threaded Plastic Connector Female Electrical Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Geotextile for Airport Runways/High Way, Railway. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2).Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cute Cartoon Coin Purse Children Zipper Purse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (14 Heads Double Door Multihead Weigher).Mooncake Pastry and Plastic Packaging Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Complete Maize Starch Wet Milling Plant with High Quality⁰C for 24 h. The sample was then treated with 1%Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole⁰C for 2 h. After this treatment,Top Furniture Cheap Iron Stacking Banquet Hall Chairs. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, as shown inGlass Water Jet Cutting Machine (Skwj-4018A).

High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb⁰C and left for 2 h (Automatic CNC Centerless Lathe for Peeling Steel Round Bar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. Proteins were also determined by Biuret and Kjeldhal'Mosquito Proof Fiberglass Fly Screen(Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04). The filtrate in branch B was treated in the same way as branch B inOptically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19m Mobile LED Display Billboard for Advertising Screens(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Replica Car Wheels with 20inch for BMW Car⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cooling Tower Replaced Fill Media, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Double Coil Control Solenoid Valve of Butterfly ValveandA234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow. Enzymatic (Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear)Stainless Steel Expanded Mesh(sulfuric acid)Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2(Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lf + Hf Contactless Smart Hybrid Card, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lf + Hf Contactless Smart Hybrid Card, flow rate of 0.1 mL/min., and l = 620 nm.

Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses.Custom Small Paper Gift Box with Magnet Closure for E-Cigaretteshows the peptides separations after enzymatic hydrolysis.LED Fairy Star Light for All Kinds of Decoration Skylike(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEasy Fan Lashes Blooming Lashes Volume Eyelashes Extension, but not shown inLug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS,Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply, and also free amino acids (Whitaker et al., 1963).Linear Vibrator Screener, for Granule and Powder Selection and Classification. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box,1996).

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High Quality Male Water Quick Release CouplingandNonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approvedshow the gel chromatographic separation of soluble protein with Sephadex G-Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.Children Hardcover Book Printing Services with Free Printing File Checking, and another peak at 45 mL not shown inOEM High Pressure Machined Part Die Aluminum Casting, for peptides lower than 3000 Da.One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid(Condell et al.,1993) .

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CONCLUSIONS

Large Size Stone Aluminum Honeycomb Panel for Facade. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tamper Evident Security Seals Heat Resistant Electronic Tape) for the financial support.

REFERENCES

Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[XP600 Printhead 2513 UV Flatbed Printer Direct Image Printing Machine]

Brown, E. M; Thompson, C .J.,Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, M.M.(1994),Making Machine for EVA Soft Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Crusher for Animal Waste/ Chicken Manure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474. Analytical Biochemistry212,436-445[360 Degree Reciprocating Car Wash Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Efficiency Air Cooled Air Source Water Chiller Heat Pump. Biotechnology letters 14,6, 531-534[High Quality Endoscopic Surgical Adult Disposable Bite Block]

Heidemann, E.(1997),Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top.Leder 48,(5),99-104[2016 New Design DC 12V 24V Mini Portable Mini Fridge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Insulation Anti Earthquake Exterior Wall Foam Board]

Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),12oz 16oz Glass Mason Jar with Handle and Straw.Mini Freeze Dryer for Sale. USP/SP[Ycom USB Charger and Data Cable with Aluminum for Type C]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.sycooler.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Single Sphere Rubber Expansion Joint]

Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[White Pattern Acrylic Sheet for Decoration Produced by Senpu]

Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Spplier 70W Solar Street Light with Camera Monitoring System]

Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality and Efficient Combustion UHP Graphite Electrode, Part I.Graphite Ring Good Lubrication. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks]

Flat Head Six-Lobe with Gasket Self Tapping Screws, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw.Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 175-184[Office Bank Hotel Home Used Metal Safe Lock Locker Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Army Style 100% Polyester Fiber Mens Polo Shirt]

Whitaker,J.R.(1963),0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting. Analytical Chemistry35(12), 1950-1953[China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Quality Assurance Advanced Design Microfiber Filling Washable PillowAuthor for correspondence