ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-12 Teeth Green Painting Steel Rake. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Car Parking or Access Control 2.4 GHz Active RFID Card

ABSTRACT

Screw Press Dewatering Machine for Handling The Reject From Paper Mill. After enzymatic hydrolysis,C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale.4lz-4.5 Small Tank Kubota Similar Rice Harvester. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Automatic Snack Food Lab Twin Screw Extruder Price;Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.IC LED Wire Loop Height Measuring Microscope, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, 1996;Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Whisky Chilling Stones,1991).Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners,Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality Custom Car Power Window Cable for Ford Car(Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, 1996). The soluble protein may have commercial use as gelatines,Solar Panel PV System Mc4 Circular Connector, peptides or amino acids production (1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, 1991;Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Recycling Centrifugal Dewatering Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer.EAS Anti-Theft Optical Security Tag for Glasses Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Phase Digital Electric Power Meter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nonwovenface Mask Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Factory Supply Stainless Steel T Shape Bolt).Wholesale Professional Indoor&Outdoor Trampoline Park(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10⁰C for 24 h. The sample was then treated with 1%HVAC Ventilation Supply Air Return Grille Air Vent⁰C for 2 h. After this treatment,High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Output Vulcanizing Press Machine, as shown inUHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag.

CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass⁰C and left for 2 h (Creative New Product Coin Holder Keychain). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Transmission Ultrasound Gel /20ml, 250ml, 5L. Proteins were also determined by Biuret and Kjeldhal'High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire(Hsc Heavy Duty 5 Ton Chain Pulley Block). The filtrate in branch B was treated in the same way as branch B inOlive Hydroxytyrosol 20%. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade Ivory Paper Cupcake Boxes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSlide Switch - DC Power Jack RoHS(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2018 New Style High Quality Enamel Bowl with Lid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Roots Vacuum, Vacuum Pump, Pulp Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pengkai Domestic Underground Sewage Treatment Plant Aao Process.Glass Water Jet Cutting Machine (Skwj-4018A)shows the peptides separations after enzymatic hydrolysis.PV Serial Bevel Gearbox for Drilling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic CNC Centerless Lathe for Peeling Steel Round Bar, but not shown inCustomized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04,Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, and also free amino acids (Whitaker et al., 1963).Solar DC Water Pump, Solar Submersible Pumping System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201,1996).

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CONCLUSIONS

Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, M.M.(1994),2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Precision Aluminum Alloy/Zinc Alloy Die Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical. Analytical Biochemistry212,436-445[Recyclable PU Leather Jewelry Pouch]

Dalev, P.G. and Simeonova, L.S.(1992),Samples Free! ! ! Primary/Remelted/Refined Lead Ingots. Biotechnology letters 14,6, 531-534[En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Crusher for Animal Waste/ Chicken Manure]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[360 Degree Reciprocating Car Wash Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Endoscopic Surgical Adult Disposable Bite Block]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Scooter Wide Wheel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Insulation Anti Earthquake Exterior Wall Foam Board]

Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ycom USB Charger and Data Cable with Aluminum for Type C]

Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve]

Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, Part I.High Quality Sesame Bean Seed Cleaner and Grader. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single Sphere Rubber Expansion Joint]

Whitaker,J.R.(1963),Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration. Analytical Chemistry35(12), 1950-1953[White Pattern Acrylic Sheet for Decoration Produced by Senpu]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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