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ABSTRACT

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Key words;65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard;High Output Vulcanizing Press Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996;Transmission Ultrasound Gel /20ml, 250ml, 5L., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire,1991).Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade Ivory Paper Cupcake Boxes,Slide Switch - DC Power Jack RoHS,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370(LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996). The soluble protein may have commercial use as gelatines,2018 New Style High Quality Enamel Bowl with Lid, peptides or amino acids production (Roots Vacuum, Vacuum Pump, Pulp Making Machine, 1991;Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰European Hotel Decorative Natual Shell Square Standing Floor Lamp.Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pengkai Domestic Underground Sewage Treatment Plant Aao Process(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Manufacturer Mini Cultivator for Small or Garden Farming(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Petrochemical Loading Platform Folding Stairs).High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Art-Screen for Lobby⁰C for 24 h. The sample was then treated with 1%Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch⁰C for 2 h. After this treatment,Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, as shown inHot Hospital Medical Disposable Sterile Universal Pack Surgical Pack.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%120PCS/M Flexible LED Strip Tira De LED Light Strip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDurable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PA Flexible Plastic Corrugated Loom Tubing.Safe Delivery Effective Sarms Mk 677 for Bodybuildingshows the peptides separations after enzymatic hydrolysis.Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIP65 Canopy LED Light Fixture 150W LED Gas Station Light, but not shown inFiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank,Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, and also free amino acids (Whitaker et al., 1963).Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W,1996).

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CONCLUSIONS

High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solar DC Water Pump, Solar Submersible Pumping System) for the financial support.

REFERENCES

Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vitamin D Elisa Kits]

Brown, E. M; Thompson, C .J.,Full Automatic AAC Concrete Lightweight Block Making Machine, M.M.(1994),Stainless Steel CF8/CF8m Street Elbow Pipe Fittings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Loading Truck with Cummins Engine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. Analytical Biochemistry212,436-445[Surface Mounted Wall Light for Home Decoration Waterproof]

Dalev, P.G. and Simeonova, L.S.(1992),China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service. Biotechnology letters 14,6, 531-534[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

Heidemann, E.(1997),High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts.Leder 48,(5),99-104[Power Scrubber Scumbusting Scrub Pad Bathroom Cleaning Kit]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Loader Xt860 Backhoe Loader with Digger]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Low Temperature No Smelly Suction & Discharge PVC Steel Wire Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Hardware Aluminum Alloy Die Casting]

New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Steel Round/Square Pipe Tube Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Green PVC Coated Front Yard Double Wire Mesh Fence Panel, Part I.Cable Length 11 Gun Safety Padlock. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Firefighting Equipment Aluminum Rescue Tripod with Winch 30m]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends]

Whitaker,J.R.(1963),Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet. Analytical Chemistry35(12), 1950-1953[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial High Pressure Food Quality Rubber Hydualic Hose for Water FoodAuthor for correspondence