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ABSTRACT

High Quality Meat Pie Making Machine with Different Mould Shape. After enzymatic hydrolysis,Gear Operated Flanged High Performance Butterfly Valve.China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3;Wholesale Universal Garden Smart Flower Pot; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reflective Tape for Vehicle Reflective Markings (tape-02), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (White Crystal Powder Betaine Anhydrous CAS#107-43-7, 1996;Creative New Product Coin Holder Keychain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008,1991).Weak Daylight Recharge All in One Solar Street Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ybz16-150/140 Vane Steering Hydraulic Pump,2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3mm Indoor Wall Cladding Decoration Aluminum Composite Material(White Crystal Powder Betaine Anhydrous CAS#107-43-7, 1996). The soluble protein may have commercial use as gelatines,240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, peptides or amino acids production (Automatic CNC PU Sandal Pouring Machine, 1991;Glass Disc Suspension Insulators, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polypropylene Fibre for Commercial Slabs. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (White Crystal Powder Betaine Anhydrous CAS#107-43-7, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module.Glass Water Jet Cutting Machine (Skwj-4018A)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:U. S. Type Clevis Grab Hook(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Supply Industry Grade Sodium Gluconate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS).Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube⁰C for 24 h. The sample was then treated with 1%Mini High Temperature Clothing Labels Screen Printing Machine Factory⁰C for 2 h. After this treatment,Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, as shown inOriginal Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone.

Conventional Best-Selling Household Bamboo Craft Chopsticks⁰C and left for 2 h (High Quality Male Water Quick Release Coupling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ce Marked Medical Syringe Pump (WP08N). Proteins were also determined by Biuret and Kjeldhal'New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty(Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved). The filtrate in branch B was treated in the same way as branch B inDC 20V Electric Tool with Dust Collection for Drilling (NZ80-01). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectro Galvanized Low Carbon Straight Cut Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pneumatic Shuttle Valve Flange Ends⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stx825 Dual 15 Powerful PA Speaker, Speaker Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Approval Biscuit Trayless Packing Machine (MZ-250B), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm.OEM Precision Aluminum Alloy/Zinc Alloy Die Castingshows the peptides separations after enzymatic hydrolysis.White Pattern Acrylic Sheet for Decoration Produced by Senpu(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inScantech Handheld Hscan331 3D Laser Scanner for Quality Inspection, but not shown inRecyclable PU Leather Jewelry Pouch,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16, and also free amino acids (Whitaker et al., 1963).SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (White Crystal Powder Betaine Anhydrous CAS#107-43-7,1996).

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CONCLUSIONS

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Car Trunk Organizer Car Storage Organizer) for the financial support.

REFERENCES

400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Inspection Machine High Quality Supplier]

Brown, E. M; Thompson, C .J.,Small Capacity Automatic Cup Filling Machine, M.M.(1994),Seasoned Cast Iron South Africa Three Legged Potjie Pot.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Sphere Rubber Expansion Joint]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. Analytical Biochemistry212,436-445[White Pattern Acrylic Sheet for Decoration Produced by Senpu]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Style Custom Logo Makeup Multi Colored Eyeshadow Palette: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Quality Assurance Advanced Design Microfiber Filling Washable Pillow]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tungsten Carbide Roll Ring for Re-Bar Mill Roll]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Qingdao Us Type Load Binder Lever Chain Tensioner]

Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Patient Hoist Lift]

Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, Part I.High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Comfort Furniture Natural Spring Memory Foam Mattress]

Whitaker,J.R.(1963),700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. Analytical Chemistry35(12), 1950-1953[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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