ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Sensitivity Ground Metal Detector Searching for Treasure. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminium Profile Bending Machine for Insulating Glass

ABSTRACT

Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws. After enzymatic hydrolysis,304 Thick Wall Pressure Vessel Heat Exchanger Pipe.High Quality Betaine Anhydrous with Best Price107-43-7. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards;Extra Heavy Wall Thickness Seamless Tubes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shrink Sleeves Labels for Packaging Labeling, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 1996;Plastic Mould Dongguan Molding Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings,1991).Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer,PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality Banded Type Tee(IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 1996). The soluble protein may have commercial use as gelatines,Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, peptides or amino acids production (Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector, 1991;Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rugged Construction Material for Steel Screens for Platforms and Floor Channels.., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FKM Rubber Gasket/Rubber O Rings in China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Inclined Waste Water Treatment Lamella Clarifier. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰33kv 100kVA Dry-Type Distribution Transformer.Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine).Ivd Component PP Barbed Threaded Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Workshop Tools 7.5 Ton Electric Winch with Dual Speed⁰C for 24 h. The sample was then treated with 1%Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment⁰C for 2 h. After this treatment,Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, as shown inHigh Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door.

2017 New Design Vinyl 3D Stone Wallpaper Factory in China⁰C and left for 2 h (Solid Carbide Single Flute End Mills Oblique Angle Cutting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Small Paper Gift Box with Magnet Closure for E-Cigarette. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Agricultural Mini Tiller Farm Wheel 62cc Cultivator. Proteins were also determined by Biuret and Kjeldhal'0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price(OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape). The filtrate in branch B was treated in the same way as branch B inKayak Rack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Efficient Online Hot Glue Dispensing Machine for Electronic Products. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automotive Sheet Metal Stamping Car Auto Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machineand2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1. Enzymatic (Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation)New Patient Hoist Lift(sulfuric acid)CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23)(IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering.12V Car Cigarette Lighter Adapter Power Extension Cordshows the peptides separations after enzymatic hydrolysis.Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBY60100C type metal shaping machine/hydraulic shaper machine, but not shown inPE100 Plasitc Pipe for Gas Water Chemcial Sewage System,0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood, and also free amino acids (Whitaker et al., 1963).1080W Most Powerful LED Flood Light Fixtures. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (IP65 Canopy LED Light Fixture 150W LED Gas Station Light,1996).

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36VandTemperature Sensor Customized Thermostat for Industrial Appliancesshow the gel chromatographic separation of soluble protein with Sephadex G-OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery.Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, and another peak at 45 mL not shown inMen′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood, for peptides lower than 3000 Da.High Quality Bucket Segment Bolt 6V0937(Condell et al.,1993) .

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Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, V_e, and K_avcalculated as discussed previously.Outdoor Server Rack Network Data Cabinet,Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube) (Reis et al.,1989;Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector.,1991;Plastic Mould Dongguan Molding Factory.,1991).Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House(1992), were lower, except for cystine and tyrosine,High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable.

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CONCLUSIONS

Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ) for the financial support.

REFERENCES

OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Charcoal Toothpaste]

Brown, E. M; Thompson, C .J.,Carbon Steel Pipe ASTM A106 A53, M.M.(1994),Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Spare Parts Ignition Coil 22448-2y001]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Peptides Powder Ghrp 2 for Muscle Building with Super Quality. Analytical Biochemistry212,436-445[High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence]

Dalev, P.G. and Simeonova, L.S.(1992),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Biotechnology letters 14,6, 531-534[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Heidemann, E.(1997),EPDM, PVC Car or Door Rubber Sealing Strip.Leder 48,(5),99-104[Ce & German Tech Mineral Wool/ Rockwool Board/Sheet/Panel Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tamper Evident Security Seals Heat Resistant Electronic Tape]

PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Black Color Tube Eyelet Lift Gas Spring.Safety Edge Sensor for Agv Automated Guided Vehicle. USP/SP[PVC Wood Grain Decorative Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Acupressure Mat Warehouse]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike]

Best Design Felt Belt Type Cooling Table/Handling Extrusion System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Document Storage Durable Metal Wire Mesh Decking for Pallet Rack]

Best Design Felt Belt Type Cooling Table/Handling Extrusion System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes]

Best Design Felt Belt Type Cooling Table/Handling Extrusion System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ycom USB Charger and Data Cable with Aluminum for Type C, Part I.Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Seashell Special Shape Magnetic Eye Shadow Empty Palette.on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage, 175-184[Cartoon Ladies Wallets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Galvanized Trench Cover]

Whitaker,J.R.(1963),Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. Analytical Chemistry35(12), 1950-1953[Dust Hood Fume Extraction Systems for Robotic Laser Cutting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr TechnologyAuthor for correspondence