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ABSTRACT

PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. After enzymatic hydrolysis,Hot Promotion Indian Market Underwear Adhesive Glue.700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, 1996;Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Aluminum Carpet Topping Stage Platform,1991).BY60100C type metal shaping machine/hydraulic shaper machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies,China Spplier 70W Solar Street Light with Camera Monitoring System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud(Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, 1996). The soluble protein may have commercial use as gelatines,Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard, peptides or amino acids production (Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, 1991;Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Masturbators Adult Sex Toys Japanese Love Doll. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale.Carbon Steel Spherical Plain Radical Bearings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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64mm Double Braid UHMWPE Tugboat Mooring Rope(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Powder Spray Booth for Metal Furniture).Rubber Soles Vulcanizing Machine/ Press Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable⁰C for 24 h. The sample was then treated with 1%Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032⁰C for 2 h. After this treatment,AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, as shown inHigh Quality 14AWG 16AWG USA spiral Power Cord to IEC C13.

Forged Steel Pressure Sealed Bonnet Globe Valve⁰C and left for 2 h (Full Automatic AAC Concrete Lightweight Block Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stable Adjustable Monitor Mounts Folding LCD Monitor Stand. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping. Proteins were also determined by Biuret and Kjeldhal'Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush(Different Size of Ring NdFeB Magnet). The filtrate in branch B was treated in the same way as branch B inWhere To Buy Sodium Hexametaphosphate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manual Double Bridge Glass Cutting Table Machine (JFQG-2620).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2018 New Design Red-CNC-006 CNC Machining Aluminum Precision. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Factory Price Hg1 Self Adjustable Pipe Tank Rotator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic CNC Centerless Lathe for Peeling Steel Round Bar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Powder Spray Booth for Metal Furniture) for the financial support.

REFERENCES

PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5083 H111]

Brown, E. M; Thompson, C .J.,3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts, M.M.(1994),Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone.The Journal of the American Leather Chemists Association (JALCA)89(7)[42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wheel Loader Transmission Gear Box/Customized Casting Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. Analytical Biochemistry212,436-445[DC Stand Fan Motor PCBA Card 24V]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine. Biotechnology letters 14,6, 531-534[Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical]

Heidemann, E.(1997),4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps.Leder 48,(5),99-104[m.yunyangcosmetic.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Single Mode Sc/APC Simplex Fiber Optic Adapter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Newest Adjustable Decline Bench Flat Weight Bench for Wholesales]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[cnmirant.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Amplifier Tube Glass Vacuum Tube (833C)]

50kg Soya Bean Seed PP Woven Bags Sacks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Iron Wire Dry Type Straight Line Wire Drawing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Large Diameter Aluminum Pipe 3004]

50kg Soya Bean Seed PP Woven Bags Sacks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12# X 14# Electric Galvanized Double Strands Barbed Wire]

50kg Soya Bean Seed PP Woven Bags Sacks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, Part I.Barricade Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence]

Whitaker,J.R.(1963),Oral Thermometer. Analytical Chemistry35(12), 1950-1953[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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