ARTICLES

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ABSTRACT

Large Stock Aerators for Shrimp Farms and Fish Pond. After enzymatic hydrolysis,750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle..Large Diameter Aluminum Pipe 3004. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory;OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Integrated Waterproof PVC Lamination Bathroom Wall Panels, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Embossed PVC Rexine Car Seat Cover Leather, 1996;Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Solar Aluminum Rail Splice Connector for Rail Extention,1991).Grade Aluminum Fly Fishing Thermometer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Single Flute End Mill Milling Cutter Tools for Wood Milling,Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sc Fiber Optic Fast Connector(Embossed PVC Rexine Car Seat Cover Leather, 1996). The soluble protein may have commercial use as gelatines,Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, peptides or amino acids production (10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1991;Watermark Approval Brass Press Equal Fitting Male Tee, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Modern Steel Structure Layer Chicken Farm House Designs., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Forged 316 Stainless Steel Marine Twisted Long D Shackle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Embossed PVC Rexine Car Seat Cover Leather, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels.Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

P4.75 Indoor Single Dual Color Advertising LED Display Billboard, Parana State,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Embossed PVC Rexine Car Seat Cover Leather, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Hole Punching Machine/ CNC Perforation MachineandMax Twintier Rb441t Using Wire Coils Tw1061t.

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Plug-in Assembly Type Straight Through Joint and Insulation Joint(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable).Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin⁰C for 24 h. The sample was then treated with 1%Quilting Machine for Muti- Needle Mattress Quilting Machine⁰C for 2 h. After this treatment,Single-Screw Plastic Extruders Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quanlity Colorful Aluminum Spacer/Standoff, as shown inHeater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A.

5g OPP Silica Gel Desiccant⁰C and left for 2 h (Solar Collector Panel for Pool Heater). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Winch With Groove Drum. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump. Proteins were also determined by Biuret and Kjeldhal'Truck Air Dryer cartridge 14bar(Stainless Steel Checkered Plate 304 316L 410 Floor Material). The filtrate in branch B was treated in the same way as branch B in600x600 Mini Led Panel Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3mm White Waterproof PVC Foam Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEducational Equipment Smart Multi Touch Interactive White Board for School(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Women Party Jewelry 45cm Sequins Choker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital SignageandHigh Freqency PCB with Blind Via. Enzymatic (Strong Aluminum Armrest Reclining Hydraulic Barber Chair)Factory SMD3528 60LEDs/M Flexible LED Strip(sulfuric acid)Hot-Press Small Continuous Diamond Saw Blade Cutting Disc(Embossed PVC Rexine Car Seat Cover Leather, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, flow rate of 0.1 mL/min., and l = 620 nm.

Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick.Top Furniture Cheap Iron Stacking Banquet Hall Chairsshows the peptides separations after enzymatic hydrolysis.Comfort Furniture Natural Spring Memory Foam Mattress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCe Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, but not shown inFactory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370,Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, and also free amino acids (Whitaker et al., 1963).4.2A Type a Type C Pd QC3.0 USB Charger. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Embossed PVC Rexine Car Seat Cover Leather,1996).

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Ppgi Material

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CONCLUSIONS

Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB) for the financial support.

REFERENCES

Customized UV Drying Heating Machine for Screen Printer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[DMX Winch LED Kinetic Lights/LED Kinetic Tube/LED Kinetic Stick]

Brown, E. M; Thompson, C .J.,99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), M.M.(1994),Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.The Journal of the American Leather Chemists Association (JALCA)89(7)[Female Header Conn Ptich 1.0mm Connector Btb]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wooden Queen/King Size Japan Sofa Bed G1162]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. Analytical Biochemistry212,436-445[Nitinol Disposable Guide Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Biotechnology letters 14,6, 531-534[0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price]

Heidemann, E.(1997),Cog Mono Round Display LCD Module.Leder 48,(5),99-104[Wallpaper Printing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Educational Equipment Whiteboard]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Products Suppliers Camlock Coupling Quick Couplings Female: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Graphite Ring Good Lubrication]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blank White Contact Ic Card Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Agrochemicals]

Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3.2m Vertical Wheelchair Elevator Platform for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Small Brown Glass Bottles]

Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins]

Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LC Multistage Pump Mechanical Seal (KLLC), Part I.Wedding Tent Cooling System Outdoor Event Air-Con Units. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB]

M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel.Prime Gi Steel Strip Galvanized Metal Strip Price, 175-184[Test Probe Multimeter Probe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer]

Whitaker,J.R.(1963),Fireproof Aluminum Curtain Wall Panel. Analytical Chemistry35(12), 1950-1953[Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball ValveAuthor for correspondence