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ABSTRACT

Geotextile for Roofs, Civil Applications. Railway Work. After enzymatic hydrolysis,Pneumatic Shuttle Valve Flange Ends.Stx825 Dual 15 Powerful PA Speaker, Speaker Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, 1996;5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter,1991).Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White Pattern Acrylic Sheet for Decoration Produced by Senpu,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends(Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, peptides or amino acids production (110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, 1991;Supply 20-110mm PP Reducing Tee for Pipe Systems, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5 Gallon Pure Water Bottle Non-Spill Cap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solid Carbide Single Flute End Mills Oblique Angle Cutting.High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Amplifier Tube Glass Vacuum Tube (833C)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (316L 10 Micron Sintered Stainless Steel Filter Disc).Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Style Custom Logo Makeup Multi Colored Eyeshadow Palette⁰C for 24 h. The sample was then treated with 1%Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending⁰C for 2 h. After this treatment,20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume, as shown inCeiling Mounted IV Stand Stainlesss Steel I V Pole.

Daytai 1550nm External Modulation Optical Transmitter 1550 -50km⁰C and left for 2 h (Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Promotion Indian Market Underwear Adhesive Glue. Proteins were also determined by Biuret and Kjeldhal'700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit(Stainless Steel Resin Trap Strainer). The filtrate in branch B was treated in the same way as branch B inm.hengyaomachinery.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Industrial Wheeled Wooden Shoe Display Fixtures for Shop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Aluminum Carpet Topping Stage Platform, pH 3.25., mobile phase B: buffer solution of 0.01 mol/BY60100C type metal shaping machine/hydraulic shaper machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor MatandFull Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies. Enzymatic (China Spplier 70W Solar Street Light with Camera Monitoring System)Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud(sulfuric acid)Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard(Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, flow rate of 0.1 mL/min., and l = 620 nm.

Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader.Drug Delivery ABS Hospital Medical Treatment Medicine Cartshows the peptides separations after enzymatic hydrolysis.Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, but not shown inLinear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet,Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory, and also free amino acids (Whitaker et al., 1963).Masturbators Adult Sex Toys Japanese Love Doll. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click,1996).

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CONCLUSIONS

Petrochemical Loading Platform Folding Stairs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Seasoned Cast Iron South Africa Three Legged Potjie Pot) for the financial support.

REFERENCES

64mm Double Braid UHMWPE Tugboat Mooring Rope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Biodegradable Disposable Printing Drinking Straws with Printed Design Patterns Wrapped Juice Straw]

Brown, E. M; Thompson, C .J.,Rubber Soles Vulcanizing Machine/ Press Machine, M.M.(1994),Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Manufacture Hydraulic Cylinder Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032. Analytical Biochemistry212,436-445[Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)]

Dalev, P.G. and Simeonova, L.S.(1992),AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe. Biotechnology letters 14,6, 531-534[Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale]

Heidemann, E.(1997),40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade.Leder 48,(5),99-104[3 Rpm Gear Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CS1363 a Standard UK 3 Pin Power Plug]

Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen.Forged Steel Pressure Sealed Bonnet Globe Valve. USP/SP[3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[on-Sale Flat Transparent Glass Jar for Sweet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stable Adjustable Monitor Mounts Folding LCD Monitor Stand: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Patented Wireless Recharge Teeth Whitening LED Light&Gel Pens Home Kit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Power Plant Circulating Water Pump & Spare Part]

CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine]

CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manual Double Bridge Glass Cutting Table Machine (JFQG-2620), Part I.2018 New Design Red-CNC-006 CNC Machining Aluminum Precision. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chenille Microfiber Mop Head]

China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Crawler Excavator Track Bolt 3p2274.SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, 175-184[Alloy 1100 3003 3105 5052 Color Coated Aluminum Sheets /Coils]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine]

Whitaker,J.R.(1963),Automatic CNC Centerless Lathe for Peeling Steel Round Bar. Analytical Chemistry35(12), 1950-1953[High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Solid Carbide Single Flute End Mills Oblique Angle CuttingAuthor for correspondence