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ABSTRACT

Stainless Steel Art-Screen for Lobby. After enzymatic hydrolysis,Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch.Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat;Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, 1996;Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Samples Free! ! ! Primary/Remelted/Refined Lead Ingots,1991).Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,120PCS/M Flexible LED Strip Tira De LED Light Strip,Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch(2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, 1996). The soluble protein may have commercial use as gelatines,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage, peptides or amino acids production (Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, 1991;ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Sesame Bean Seed Cleaner and Grader., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pet Strapping Band Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label.Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PA Flexible Plastic Corrugated Loom Tubing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double BowlAlcalase2.5 L DX,Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)3-Way T Type Elbow Quick Connect Pneumatic Fitting(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

China Superior Quality Soya Protein Food Production Machine Line, Parana State,High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light.Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3-Layer PE Coating Production LineandGreen PVC Coated Front Yard Double Wire Mesh Fence Panel.

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Custom Army Style 100% Polyester Fiber Mens Polo Shirt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Super Bass Music TV).E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Twisted Annealed Wire for Binding⁰C for 24 h. The sample was then treated with 1%Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid⁰C for 2 h. After this treatment,Wholesale Fashion Jewellery Lady Eardrop Earrings for Women. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, as shown inLow Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box.

Full Automatic AAC Concrete Lightweight Block Making Machine⁰C and left for 2 h (Twisted Annealed Wire for Binding). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel CF8/CF8m Street Elbow Pipe Fittings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. Proteins were also determined by Biuret and Kjeldhal'China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service(Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse). The filtrate in branch B was treated in the same way as branch B inHigh Water Efficiency Single-Suction Pump Cantilever Cement Mortar Sewage Pumping Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Low Volume Production Aluminum CNC Machining Auto Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carbon Steel Round/Square Pipe Tube Production Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Green PVC Coated Front Yard Double Wire Mesh Fence Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Price High Quality Automatic Egg Tray Egg Carton Making Production MachineandCable Length 11 Gun Safety Padlock. Enzymatic (Canvas School College Backpack/Bookbags for Girls/Students/Women)Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles(sulfuric acid)Women′s Seamless Strapless Bra(2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, flow rate of 0.1 mL/min., and l = 620 nm.

300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooksshows the peptides separations after enzymatic hydrolysis.Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inReflective Tape for Vehicle Reflective Markings (tape-02), but not shown inFactory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses,(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station, and also free amino acids (Whitaker et al., 1963).off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays,1996).

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CONCLUSIONS

Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet) for the financial support.

REFERENCES

Ride on Toys Riding Animals in The Mall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Self Drilling Screw]

Brown, E. M; Thompson, C .J.,Finely Processed Pneumatic Operated Globe Valve, M.M.(1994),Color Coated Corrugated Galvanized Roofing Steel Plate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality Banded Type Tee]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. Analytical Biochemistry212,436-445[Natural OEM Private Label Customized Skin Care Face Serum]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. Biotechnology letters 14,6, 531-534[Black Plastic Handle Stainless Steel Kitchen Gadget]

Heidemann, E.(1997),Precision Diamond Wire Saw Rope for Cutting Concrete Stone.Leder 48,(5),99-104[www.baotn.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply]

Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty.Blince High Torque OMR Hydro Motor for Injection Moulding Machine. USP/SP[Best Shatterproof Gold Rim Wine Glasses]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer Supply Durable Grey Color Non-Slip EVA Mat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dental Stereo Microscope]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Single-Screw Plastic Extruders Machine]

TV Tower Antenna Supports, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[50Hz/60Hz Low Frequency Home Power Inverter 5000W]

TV Tower Antenna Supports,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Besen Portable EV Fast Charger  with Schuko Plug]

TV Tower Antenna Supports, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Retractable Multi Core Cable Reel, Part I.New Xcm 120 Asphalt Mixing Plant for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks]

Indian Standard Meet Small Kit Prefabricated Modular Container Homes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings.LED Indoor Light Linear Light Aluminium LED Profile, 175-184[Kinds Melamine Faced Plywood MDF Chipboard Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards]

Whitaker,J.R.(1963),50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price. Analytical Chemistry35(12), 1950-1953[Industrial Sausage Meat Smoker Oven/Smokerhouse Oven]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Various Bridge NdFeB Motor Magnets with High PerformanceAuthor for correspondence