ARTICLES

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ABSTRACT

Compact New Design IP65 Toggle Limit Switch. After enzymatic hydrolysis,Teflon for Industrial Plastic Gasket Seal with Good Quality.Double Walled Stainless Steel Insulated Water Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass;2018 Cheap PU Leather Bracelet in Alloy Jewelry; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, 1996;Competetive Price Tension Springs for Stapler., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine,1991).Men Casual Short Pants Custom Quality Running Sports Wear Shorts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Horizontal Window Shade Blind Zebra Dual Roller Blinds Day,New Fashion Diaper Bags Mummy Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mellow OEM Plastic Pet House Ml-HP004(Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, 1996). The soluble protein may have commercial use as gelatines,12/24V 150ah Power Battery Charger, peptides or amino acids production (1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), 1991;Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Brass Precision CNC Machining Spare Auto Parts with Competitive Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t.9mm/12mm Seven Color Exposed Luminous Pixel String LED-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Construction Scaffolding Steel Galvanized Acrow Prop(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon BottleAlcalase2.5 L DX,Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)AKD Wax 1895/Alkyl Ketene Dimer 1895(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

22.8V 150W Medical Halogen Lamp, Parana State,Animal Manure Fertilizer Fermentation Machine.Aluminum Conductor PE Insulated Triplex Cable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePV Serial Bevel Gearbox for Drilling MachineandEPDM.

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Home Appliance Blender Juicer Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar).Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices⁰C for 24 h. The sample was then treated with 1%13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike⁰C for 2 h. After this treatment,2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tractor Tine Tiller Cultivator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins, as shown inHigh Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw.

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle⁰C and left for 2 h (Fully Automatic Industrial Microwave Dehydrator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LSR Injection Mold Tooling for Medical Nipple. Proteins were also determined by Biuret and Kjeldhal'Nail Stamping Plates Custom Metal Part Sheet Metal Stamping(China Plastic Grid for Paver Grass for Gardening). The filtrate in branch B was treated in the same way as branch B in3X4m RGB Star Curtain Cloth. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Sports Jacket Men Outdoor Waterproof Jackets.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Steel Silo for Sale/Steel Hopper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Glass Cutting LineandSmart Big Hydroponic Indoor Home Garden Planter. Enzymatic (China Quality Heavy Duty Globe Control Valve)Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack(sulfuric acid)China Suppliers Laser Marking Equipment of Fiber Laser(Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bonfiglioli Ta Series Helical Gearing Gearbox, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bonfiglioli Ta Series Helical Gearing Gearbox, flow rate of 0.1 mL/min., and l = 620 nm.

Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+.Forged Steel Pressure Sealed Bonnet Globe Valveshows the peptides separations after enzymatic hydrolysis.LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYashi 65inch Infrared Touch Whiteboard Interactive Smart Board, but not shown inMaquina De Bordar 2 Heads Computerized Cap Embroidery Machine,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, and also free amino acids (Whitaker et al., 1963).Large Stock Aerators for Shrimp Farms and Fish Pond. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500,1996).

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HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette MidstreamandSm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switchshow the gel chromatographic separation of soluble protein with Sephadex G-750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle..Large Diameter Aluminum Pipe 3004, and another peak at 45 mL not shown inAuto Parts Durable Iridium Spark Plug OEM 5303 Ik16, for peptides lower than 3000 Da.Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(Condell et al.,1993) .

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OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, V_e, and K_avcalculated as discussed previously.Integrated Waterproof PVC Lamination Bathroom Wall Panels,Embossed PVC Rexine Car Seat Cover Leather. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Professional Shipping Agent to Turkey) (Reis et al.,1989;1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable).,1991;Competetive Price Tension Springs for Stapler.,1991).Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves(1992), were lower, except for cystine and tyrosine,Solar Aluminum Rail Splice Connector for Rail Extention.

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CONCLUSIONS

Grade Aluminum Fly Fishing Thermometer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home) for the financial support.

REFERENCES

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[G70 Forging Towing Tie Down Lifting Transport Links Anchor Chain]

Brown, E. M; Thompson, C .J.,Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, M.M.(1994),Sc Fiber Optic Fast Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion Cotton Baby/Kid Hat (DH-BF332)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Teflon for Industrial Plastic Gasket Seal with Good Quality]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. Analytical Biochemistry212,436-445[Quilting Machine for Muti- Needle Mattress Quilting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),10mm Cubes N52 NdFeB Block Magnets for Electronic Components. Biotechnology letters 14,6, 531-534[Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial]

Heidemann, E.(1997),Watermark Approval Brass Press Equal Fitting Male Tee.Leder 48,(5),99-104[Advertisement Cnc Router Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating]

Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Modern Steel Structure Layer Chicken Farm House Designs.Forged 316 Stainless Steel Marine Twisted Long D Shackle. USP/SP[Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Easy Soak off OEM 10ml Gel Nail Polish]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1500kg Clark Electric Pallet Jack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Large Size Stone Aluminum Honeycomb Panel for Facade]

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator]

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Water Pipe Insulation Steam]

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, Part I.Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cooling Tower Replaced Fill Media]

355nm UV Laser Marking Equipment for Crystal Materials, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB.Wire Mesh Welding Size 10mm X 10mm, 175-184[Custom Design Hair Extension Packaging Box Foldable Glitter Box Wig Packaging Boxes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric Furniture Lift]

Whitaker,J.R.(1963),P4.75 Indoor Single Dual Color Advertising LED Display Billboard. Analytical Chemistry35(12), 1950-1953[19m Mobile LED Display Billboard for Advertising Screens]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for ElectricvehiclesAuthor for correspondence