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ABSTRACT

Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. After enzymatic hydrolysis,Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed.Wooden Queen/King Size Japan Sofa Bed G1162. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, 1996;Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation,1991).Dust Hood Fume Extraction Systems for Robotic Laser Cutting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector,Nordic Solid Wood Dining Chair/Smile Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet(Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, 1996). The soluble protein may have commercial use as gelatines,Experienced and Professional Plastic Helmet Mold Manufacturer, peptides or amino acids production (Private Label OEM Best Cute Waterproof Lip Pluming Gloss, 1991;Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tent Windows Use China Made PVC Transparent Clear Mesh Tarp.13 Oz Flex Banner PVC Frontlit Banner Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Unique Best Quality Copper Home Alcohol Distiller for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector).Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector⁰C for 24 h. The sample was then treated with 1%Drive Travel Car Inflatable Bed⁰C for 2 h. After this treatment,Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China PCBA Supplier Custom Game Controller PCB's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)TPU TPR Rubber Band Plastic Extruder Machine, as shown inCorrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve.

Wheel Loader Transmission Gear Box/Customized Casting Parts⁰C and left for 2 h (Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Spare Parts Ignition Coil 22448-2y001. Proteins were also determined by Biuret and Kjeldhal'3D Good Quality Laser Engraving Machine for Souvenirs Shop( Portable Concrete Mixing Plant). The filtrate in branch B was treated in the same way as branch B inSapphire And Pearl Earrings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReverse Flow Configuration Axial Fan(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Spindle Micro CNC Lathe Ck0640, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V) for the financial support.

REFERENCES

ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[96mm PP and PE Combination Mooring Rope]

Brown, E. M; Thompson, C .J.,China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, M.M.(1994),XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe.The Journal of the American Leather Chemists Association (JALCA)89(7)[CAD Cam Dental Machine 3D Dental Model Printer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gear Operated Flanged High Performance Butterfly Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. Analytical Biochemistry212,436-445[PE Coated Paper for Salt Sugar Sachets Paper Food Grade]

Dalev, P.G. and Simeonova, L.S.(1992),Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061). Biotechnology letters 14,6, 531-534[Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Die Aluminum Castings]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Cheap Price Disposable IV Infusion Set with Precise Filter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Constant Humidity Cabinet Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100% Cotton Face Mask in Guangzhou]

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Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disposable Clear Take Away Plastic Salad Bowl Forming Machine, Part I.Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette]

Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge.Carbon Stee Semless Pipe (ASTM A106 Gr B), 175-184[Fashion Luxury Lady Designer Tassel Bags]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

Whitaker,J.R.(1963),OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale. Analytical Chemistry35(12), 1950-1953[Belt Conveyor in Heavy Duty Industry for Mining]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Solar Aluminum Rail Splice Connector for Rail ExtentionAuthor for correspondence