ARTICLES

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ABSTRACT

SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit. After enzymatic hydrolysis,Automatic CNC Centerless Lathe for Peeling Steel Round Bar.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ck6150 High Precision Metal Machining Horizontal CNC Lathe;Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, 1996;Factory Custom Round Aluminum Tube 5154 5454 5652 5086., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Newest Adjustable Decline Bench Flat Weight Bench for Wholesales,1991).PVC Wood Grain Decorative Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer,LED UV Portable Toothbrush Sterilizer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding(High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, 1996). The soluble protein may have commercial use as gelatines,American NEMA Straight Blade Plug 30A 125V/250V Power Plug, peptides or amino acids production (10in 1500lb Pressure Seal Forged Steel Swing Check Valve, 1991;Custom Printed/Embossed Black Earring Packaging Cards, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mug Printing Machine Heat Press Promotion Brazil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automotive OEM Copper Wire Harness and Connectors for Industry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692).LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sale Loading Truck with Cummins Engine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay).Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50kg Soya Bean Seed PP Woven Bags Sacks⁰C for 24 h. The sample was then treated with 1%Iron Wire Dry Type Straight Line Wire Drawing Machine⁰C for 2 h. After this treatment,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Barricade Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, as shown inMen Casual Short Pants Custom Quality Running Sports Wear Shorts.

Fully Automatic Industrial Microwave Dehydrator⁰C and left for 2 h (Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Oral Thermometer. Proteins were also determined by Biuret and Kjeldhal'Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)( Injection Molding). The filtrate in branch B was treated in the same way as branch B inAnionic Polyacrylamide Water Treatment Polymer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wood Fireplace Double Skin Chimney Flue Pipes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Sensitivity Ground Metal Detector Searching for Treasure(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/304 Thick Wall Pressure Vessel Heat Exchanger Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Betaine Anhydrous with Best Price107-43-7, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

12/24V 150ah Power Battery ChargerandFM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards. Enzymatic (Extra Heavy Wall Thickness Seamless Tubes)Shrink Sleeves Labels for Packaging Labeling(sulfuric acid)IP65 Canopy LED Light Fixture 150W LED Gas Station Light(High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Mould Dongguan Molding Factory, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Mould Dongguan Molding Factory, flow rate of 0.1 mL/min., and l = 620 nm.

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Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles, V_e, and K_avcalculated as discussed previously.Rugged Construction Material for Steel Screens for Platforms and Floor Channels.,FKM Rubber Gasket/Rubber O Rings in China. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line) (Reis et al.,1989;10in 1500lb Pressure Seal Forged Steel Swing Check Valve.,1991;Factory Custom Round Aluminum Tube 5154 5454 5652 5086.,1991).Inclined Waste Water Treatment Lamella Clarifier(1992), were lower, except for cystine and tyrosine,33kv 100kVA Dry-Type Distribution Transformer.

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CONCLUSIONS

Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker) for the financial support.

REFERENCES

Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[En31 Suj2 Gcr15 SAE52100 L3 Bearing Steel Round Plate Flat]

Brown, E. M; Thompson, C .J.,5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, M.M.(1994),Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).The Journal of the American Leather Chemists Association (JALCA)89(7)[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . Analytical Biochemistry212,436-445[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Dalev, P.G. and Simeonova, L.S.(1992),China Manufacturer Export Copper Foot Tap/Valve (HFV-001). Biotechnology letters 14,6, 531-534[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

Heidemann, E.(1997),Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro.Leder 48,(5),99-104[Mini Square Trampoline]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple]

2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PVC Corrugated or Smooth Surface Helix Suction Water Hose.Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts. USP/SP[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ivd Component PP Barbed Threaded Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ERW Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Trade Assurance Beautiful Silk Lace Closure]

Workshop Tools 7.5 Ton Electric Winch with Dual Speed, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

Workshop Tools 7.5 Ton Electric Winch with Dual Speed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549]

Workshop Tools 7.5 Ton Electric Winch with Dual Speed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, Part I.500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Kiet Hydraulic Pipe Bender Machine]

RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2017 New Design Vinyl 3D Stone Wallpaper Factory in China.Custom Small Paper Gift Box with Magnet Closure for E-Cigarette, 175-184[Marine Titanium Plate Heat Exchangers for Lubricating Oil Cooler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Disposable Sugarcane Pulp Paper Bowl 500ml]

Whitaker,J.R.(1963),Agricultural Mini Tiller Farm Wheel 62cc Cultivator. Analytical Chemistry35(12), 1950-1953[industrial aluminum foil roll 8011 8079 1235]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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