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ABSTRACT

PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. After enzymatic hydrolysis,Bending Shrapnel.12V Car Cigarette Lighter Adapter Power Extension Cord. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals;Water Bed, Water Slide, Water Roller for Indoor Playground; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Besen Portable EV Fast Charger  with Schuko Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, 1996;1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,1991).Oil Wax Furniture PVC Leather (HONGJIU-498#). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine,M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China High Efficiency Automatic Glue Dispensing Machine with Factory Price(Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, 1996). The soluble protein may have commercial use as gelatines,CNC Machining Mechanical Parts Fabrication Service, peptides or amino acids production (Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, 1991;Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electronic Pulse Gas Stove Burner Igniter Transformer Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Turned Aluminum Anodized Auto Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Commercial Vegetable Mushroom Coconut Meat Drying Machine.4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Headband Big Headphone EVA Bag Tool Case Specialized Storage Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bathroom Makeup LED Mirror Glass with Light, Parana State,Hot Sale Electrical Motors Small for Home Appliance.Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWind Protection Screen. Wind Dust NettingandAuto Spare Parts Ignition Coil 22448-2y001.

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Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence).White and Green Board, School Writing Board, School Furniture (GT-76C)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Current Precise LED Digital Ammeter⁰C for 24 h. The sample was then treated with 1%Automatic Drinking Mineral Water Filling Line/Water Bottling Line⁰C for 2 h. After this treatment,Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP Polypropylene Two Layer Film Blowing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Sugarcane Pulp Paper Bowl 500ml, as shown inBubule 17 Inch PP Carry on Kids Travel Luggage for Children.

Flat Roof Insulation Board Heat Insulation Board⁰C and left for 2 h (Tamper Evident Security Seals Heat Resistant Electronic Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Female Threaded Tee T Shape Pipe Fittings. Proteins were also determined by Biuret and Kjeldhal'Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection(PVC Wood Grain Decorative Sheet). The filtrate in branch B was treated in the same way as branch B inBakelite Vulcanization Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Natural OEM Private Label Customized Skin Care Face Serum, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Boat Deck Drainage Snow Rubber Flooring/Standing Desk MatandAir-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. Enzymatic (Automatic Screw Press Dewatering Machine for Cassava Residue)Outdoor Indoor Football Soccer Goal Post Net for Kids(sulfuric acid)Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear(Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Threaded Locking Circular Connectors M16 680. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Precision Aluminum Alloy/Zinc Alloy Die Casting) for the financial support.

REFERENCES

Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solar Wall Lamp]

Brown, E. M; Thompson, C .J.,Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, M.M.(1994),Disposable Sterile Safety Blood Lancet.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Plastic Pump Cosmetic Packaging as Airless Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plush Comfort Spring Mattress Cheap Price for Wholesale. Analytical Biochemistry212,436-445[Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool]

Dalev, P.G. and Simeonova, L.S.(1992),3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss. Biotechnology letters 14,6, 531-534[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

Heidemann, E.(1997),Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway.Leder 48,(5),99-104[Sanitary SS304/SS316L Pneumatic/Manual Welded /Clamp /Threaded Male Butterfly Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 400A]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SGS 40L Lab Use Chocolate Refiner Conching Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reducing Pipe Water Flange Cam Lock Rotary Steel Male Threaded Press Fitting Equal Schedule 40 Quick Female Fittings Bushing Stainless Slip Inox Coupling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Waterproof LED Flash Shoe Light Crystal Epoxy Resin, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Shopping Paper Bag for Clothing Package-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Waterproof LED Flash Shoe Light Crystal Epoxy Resin,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Waterproof LED Flash Shoe Light Crystal Epoxy Resin, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Qingdao Us Type Load Binder Lever Chain Tensioner, Part I.Stainless Steel Elbow SS304 SS316L. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Whitaker,J.R.(1963),14 Heads Double Door Multihead Weigher. Analytical Chemistry35(12), 1950-1953[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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