ARTICLES

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ABSTRACT

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer. After enzymatic hydrolysis,Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter.Cjx2-F (LC1-F) AC Magnetic Contactor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal;Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum/Aluminium Extrusion Profile Housing for LED Light, 1996;Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Mode Sc/APC Simplex Fiber Optic Adapter,1991).120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine,Abrasive 6 Sanding Disc for Automotive Industry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine(Aluminum/Aluminium Extrusion Profile Housing for LED Light, 1996). The soluble protein may have commercial use as gelatines,2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, peptides or amino acids production (Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm, 1991;Single Sphere Rubber Expansion Joint, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cold Press Circular Wood Cutting Machine Blade., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Trench Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum/Aluminium Extrusion Profile Housing for LED Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course.Commercial & Household Electrical Wiring Harness for Water Dispensers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wicker Flower Baskets for Planter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light).Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle⁰C for 24 h. The sample was then treated with 1%Customized Thickness EVA High Density Foam EVA Roll⁰C for 2 h. After this treatment,Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carbide Mould Parts Moulding Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Compression Mens Customized Gym Shorts Sportswear, as shown inWear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.

Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip⁰C and left for 2 h (China Factory Organic Ethyl Ascorbic Acid). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder. Proteins were also determined by Biuret and Kjeldhal'P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display(316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube). The filtrate in branch B was treated in the same way as branch B in6W off Grid Solar Power System Solar System Generator for Indoor and Outdoor Use. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Furniture Lift.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring.Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cutershows the peptides separations after enzymatic hydrolysis.Hot Sell 100% Seal Hexagon Glass Jam Jar(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10mm Cubes N52 NdFeB Block Magnets for Electronic Components, but not shown inHigh Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc,Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping, and also free amino acids (Whitaker et al., 1963).High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum/Aluminium Extrusion Profile Housing for LED Light,1996).

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CONCLUSIONS

Precision Casting Factory Foundry OEM Ductile Iron Sand Casting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Engine Parts Suspension Control Arm OEM 54501-Jg00b) for the financial support.

REFERENCES

Hot Sale Electrical Motors Small for Home Appliance, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Yellow Shielded Wire Solder Ring in The Protective Tube Heat Shrinkable Tube Hot Melt Waterproof Intermediate Terminal Connector Sst-S41 4-6mm2]

Brown, E. M; Thompson, C .J.,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, M.M.(1994),Bending Shrapnel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Group 152cm Picnic Folding in Half Table]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Extra Heavy Wall Thickness Seamless Tubes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12V Car Cigarette Lighter Adapter Power Extension Cord. Analytical Biochemistry212,436-445[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Dalev, P.G. and Simeonova, L.S.(1992),Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals. Biotechnology letters 14,6, 531-534[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

Heidemann, E.(1997),Water Bed, Water Slide, Water Roller for Indoor Playground.Leder 48,(5),99-104[Farm Hay Rake Tine Garden Tractor Hay Rake Tender]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shengya Steel Concrete Electric Pole Mould]

Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Besen Portable EV Fast Charger  with Schuko Plug.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. USP/SP[Travel Refillable Perfume Atomizer Spray Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White Marble]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single Phase Digital Electric Power Meter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steering Units Hydraulic Orbital Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Oil Wax Furniture PVC Leather (HONGJIU-498#)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, Part I.M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

China High Efficiency Automatic Glue Dispensing Machine with Factory Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining Mechanical Parts Fabrication Service.Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, 175-184[New Look Women′s Hoodies with Logo in Grey]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Grip Wood Handle Shovel]

Whitaker,J.R.(1963),Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. Analytical Chemistry35(12), 1950-1953[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6Author for correspondence