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ABSTRACT

Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer. After enzymatic hydrolysis,EAS Anti-Theft Optical Security Tag for Glasses Bottle.Single Phase Digital Electric Power Meter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly;2W 100lm LED Solar Lawn Light for Garden or Yard Lighting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (28mm China High Quality Supplier Screw Cannon Cap for Bottle, 1996;Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure,1991).Nonwovenface Mask Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Professional Indoor&Outdoor Trampoline Park,Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HVAC Ventilation Supply Air Return Grille Air Vent(28mm China High Quality Supplier Screw Cannon Cap for Bottle, 1996). The soluble protein may have commercial use as gelatines,High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, peptides or amino acids production (65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard, 1991;High Output Vulcanizing Press Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Transmission Ultrasound Gel /20ml, 250ml, 5L. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (28mm China High Quality Supplier Screw Cannon Cap for Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire.Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade Ivory Paper Cupcake Boxes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine).IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%European Hotel Decorative Natual Shell Square Standing Floor Lamp⁰C for 24 h. The sample was then treated with 1%Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT⁰C for 2 h. After this treatment,Pengkai Domestic Underground Sewage Treatment Plant Aao Process. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PV Serial Bevel Gearbox for Drilling Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, as shown inIndian Standard Meet Small Kit Prefabricated Modular Container Homes.

Solar DC Water Pump, Solar Submersible Pumping System⁰C and left for 2 h (500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow. Proteins were also determined by Biuret and Kjeldhal'Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V(High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass). The filtrate in branch B was treated in the same way as branch B inBags Accessory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Mini Cultivator for Small or Garden Farming.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Art-Screen for Lobby(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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120PCS/M Flexible LED Strip Tira De LED Light Strip.Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressorshows the peptides separations after enzymatic hydrolysis.Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Efficient Online Hot Glue Dispensing Machine for Electronic Products, but not shown inHigh Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml,Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, and also free amino acids (Whitaker et al., 1963).Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (28mm China High Quality Supplier Screw Cannon Cap for Bottle,1996).

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CONCLUSIONS

Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2018 New Style High Quality Enamel Bowl with Lid) for the financial support.

REFERENCES

Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiberglass Jacket Thermal Insulation High Temperature Fiberglass Cloth Welding Protection Fire Curtain Vermiculite Coated Fiberglass Fabrics]

Brown, E. M; Thompson, C .J.,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, M.M.(1994),3-Way T Type Elbow Quick Connect Pneumatic Fitting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Men′s High Quality Mercerised Cotton Business Dress Socks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[900kg Pneumatic Motorcycle Lift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Superior Quality Soya Protein Food Production Machine Line. Analytical Biochemistry212,436-445[Mug Printing Machine Heat Press Promotion Brazil]

Dalev, P.G. and Simeonova, L.S.(1992),Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. Biotechnology letters 14,6, 531-534[Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump]

Heidemann, E.(1997),Custom Army Style 100% Polyester Fiber Mens Polo Shirt.Leder 48,(5),99-104[Polyvinyl Alcohol Pva Granules Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2W 100lm LED Solar Lawn Light for Garden or Yard Lighting]

Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator.Twisted Annealed Wire for Binding. USP/SP[Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Colorful Electric English Funny Music Story Module Printing Book]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Twist Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ybz16-150/140 Vane Steering Hydraulic Pump]

Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment]

Wholesale Fashion Jewellery Lady Eardrop Earrings for Women,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, Part I.Full Automatic AAC Concrete Lightweight Block Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding.China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, 175-184[6 Person Office Workstation]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Whitaker,J.R.(1963),High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. Analytical Chemistry35(12), 1950-1953[TV Tower Antenna Supports]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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