ARTICLES

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ABSTRACT

New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. After enzymatic hydrolysis,Carbon Steel Round/Square Pipe Tube Production Line.Green PVC Coated Front Yard Double Wire Mesh Fence Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cable Length 11 Gun Safety Padlock;Canvas School College Backpack/Bookbags for Girls/Students/Women; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Women′s Seamless Strapless Bra, 1996;Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm,1991).Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station,off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes(Women′s Seamless Strapless Bra, 1996). The soluble protein may have commercial use as gelatines,Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, peptides or amino acids production (Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, 1991;Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20 Experiences on Plastic Injection Moulding Service., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wind Protection Screen. Wind Dust Netting, Wind Dust Net.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-24K Gold Rolling Paper Shine Papers Cigarette Paper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Women′s Seamless Strapless Bra, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Two - Way Double Row Thrust Tapered Roller Bearings.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ride on Toys Riding Animals in The Mall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Precision Diamond Wire Saw Rope for Cutting Concrete Stone, Parana State,Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Women′s Seamless Strapless Bra, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)andChina High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps.

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Blince High Torque OMR Hydro Motor for Injection Moulding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (UV Resistant Durable HDPE Reinforced Geomembrane HDPE).6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%TV Tower Antenna Supports⁰C for 24 h. The sample was then treated with 1%Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring⁰C for 2 h. After this treatment,Retractable Multi Core Cable Reel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Xcm 120 Asphalt Mixing Plant for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Indian Standard Meet Small Kit Prefabricated Modular Container Homes, as shown inFlat Prefab Shipping Container Home Prefabricated Modular Pack Movable House.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Workbench Anti-Static Table ESD Natural Rubber Table Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av316L 10 Micron Sintered Stainless Steel Filter Disc(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Abstract Light and Shadow Metal Oil Painting for Deco⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheapest Container Living House/Office/Kiosk, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Threaded Locking Circular Connectors M16 680andSpin Centrifugal Demister Separator or Column Internal. Enzymatic (Baby Wet Wipe Machine)Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump(sulfuric acid)Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric(Women′s Seamless Strapless Bra, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%360 Degree Reciprocating Car Wash Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%360 Degree Reciprocating Car Wash Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Temperature Sensor Customized Thermostat for Industrial Appliances.South Africa Standards Solar Power Variable Message Sign LED Traffic Signshows the peptides separations after enzymatic hydrolysis.Custom Die Aluminum Castings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWedding Tent Cooling System Outdoor Event Air-Con Units, but not shown inLC Multistage Pump Mechanical Seal (KLLC),High Quality Meat Pie Making Machine with Different Mould Shape, and also free amino acids (Whitaker et al., 1963).Gear Operated Flanged High Performance Butterfly Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Women′s Seamless Strapless Bra,1996).

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Full Viton Coated Half Shaft Wafer Butterfly ValveandHotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)show the gel chromatographic separation of soluble protein with Sephadex G-China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck.Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, and another peak at 45 mL not shown inNail Stamping Plates Custom Metal Part Sheet Metal Stamping, for peptides lower than 3000 Da.Wholesale Universal Garden Smart Flower Pot(Condell et al.,1993) .

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CONCLUSIONS

Ybz16-150/140 Vane Steering Hydraulic Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting) for the financial support.

REFERENCES

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shredder Machinery Used for NPK Compound Urea Fertilizer Production]

Brown, E. M; Thompson, C .J.,3mm Indoor Wall Cladding Decoration Aluminum Composite Material, M.M.(1994),240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Many Styles Fancy Wine Display Rack (6532)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic CNC PU Sandal Pouring Machine. Analytical Biochemistry212,436-445[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),Glass Disc Suspension Insulators. Biotechnology letters 14,6, 531-534[4 Gang 1 Way Electrical Fitting Ce Certificate]

Heidemann, E.(1997),2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator.Leder 48,(5),99-104[m.let-dcpump.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale High Speed Mixer Blender for Extruder Machine]

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.Polypropylene Fibre for Commercial Slabs. USP/SP[Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2017 New Design Vinyl 3D Stone Wallpaper Factory in China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HD LED Video Wall Color TV Indoor SMD P5 LED Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity]

Glass Water Jet Cutting Machine (Skwj-4018A), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),U. S. Type Clevis Grab Hook-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale]

Glass Water Jet Cutting Machine (Skwj-4018A),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag]

Glass Water Jet Cutting Machine (Skwj-4018A), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, Part I.Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Maleic Anhydride CAS 108-31-6]

Car Trunk Organizer Car Storage Organizer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Cotton Baby/Kid Hat (DH-BF332).Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, 175-184[Large Supply High Purity 99.95% 99.99% Metal Evaporation Materials Tantalum]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004]

Whitaker,J.R.(1963),OEM Round Stainless Steel Fruit Plate Tray. Analytical Chemistry35(12), 1950-1953[One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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