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ABSTRACT

Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse. After enzymatic hydrolysis,Super Potassium Humate Shiny Flakes.4K Giant Whiteboard Interactive Panel for University Whiteboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25;Electric Massage Bed Pedicure Chair with Double Dust Collector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Adjustable Solar Mounting Kits (GD1059), 1996;Bgf-800 BOPP Dry Laminating Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Test-630 Automatic Protective Relay Tester,1991).Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tpdl Belt Press Sludge Equipment with Slaughtering Machine,3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,The Most Demanded Products Fluorescence Microscope Camera(Adjustable Solar Mounting Kits (GD1059), 1996). The soluble protein may have commercial use as gelatines,Low Price Promotion Soda Ash Light to Papermaking, peptides or amino acids production (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, 1991;HOWO 420HP Rhd Tractor Truck Head, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Adjustable Solar Mounting Kits (GD1059), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb.Gaint Inflatable Animal Slide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White 4*8FT 18mm PVC Foam Board for Cabinets Use(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Carbon Steel Equal Tee for Connection of Pipe, Parana State,Wholesale Mini School Backpack for Girls.Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Adjustable Solar Mounting Kits (GD1059), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCommercial Vegetable Mushroom Coconut Meat Drying MachineandSolid Steel Hotel Safe (G-42BD classics) for Hotel.

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Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss).Steel Stacking Restaurant Wedding Chair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rutile Anatase Titanium Dioxide for Coating and Paints⁰C for 24 h. The sample was then treated with 1%Laser Cutting Machine for Sale for Die Shops From Yitai Die Making⁰C for 2 h. After this treatment,Di003 Environmental Protection Against The Stench Floor Drain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vacuum Type Single Facer Corrugator, as shown inIP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector.

Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken⁰C and left for 2 h (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. Proteins were also determined by Biuret and Kjeldhal'125mm Wet Diamond Pad for Polishing Marble(Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes). The filtrate in branch B was treated in the same way as branch B inCustomized Rattan Basket Wicker Willow Storage Basket Laundry Basket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%40W Integrated Solar Street Lighting LED for Park. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFireproof DMX Wedding Stage Curtain P18 RGB Video Curtain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Checkered Plate 304 316L 410 Floor Material⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Feed Storage OEM Grain Storage Silo 1cbm to 200cbmandScantech Handheld Hscan331 3D Laser Scanner for Quality Inspection. Enzymatic (Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods)Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports(sulfuric acid)Sport Anti Vibration Hiking Safety Shoes(Adjustable Solar Mounting Kits (GD1059), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, flow rate of 0.1 mL/min., and l = 620 nm.

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Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, V_e, and K_avcalculated as discussed previously.Cute Cartoon Coin Purse Children Zipper Purse,Garment Lace Coating Machine Automatic Silicone Coating Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (IP65 Canopy LED Light Fixture 150W LED Gas Station Light) (Reis et al.,1989;Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle.,1991;Bgf-800 BOPP Dry Laminating Machine.,1991).2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)(1992), were lower, except for cystine and tyrosine,Tamper Evident Security Seals Heat Resistant Electronic Tape.

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CONCLUSIONS

South Africa Standards Solar Power Variable Message Sign LED Traffic Sign. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Power Plant Circulating Water Pump & Spare Part) for the financial support.

REFERENCES

2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cement Tiles Manufacturing Machines]

Brown, E. M; Thompson, C .J.,Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels, M.M.(1994),Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hotbar Reflow Machine for FPC of Camera]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mooncake Pastry and Plastic Packaging Box. Analytical Biochemistry212,436-445[Food Grade Beverage Clear PVC Tubing Hose]

Dalev, P.G. and Simeonova, L.S.(1992),Complete Maize Starch Wet Milling Plant with High Quality. Biotechnology letters 14,6, 531-534[Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r]

Heidemann, E.(1997),Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole.Leder 48,(5),99-104[Audio Converter Adapter Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker]

12# X 14# Electric Galvanized Double Strands Barbed Wire, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Top Furniture Cheap Iron Stacking Banquet Hall Chairs.Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle. USP/SP[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Colored Multiwall Polycarbonate Hollow Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Loading Truck with Cummins Engine]

High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Surface Mounted Wall Light for Home Decoration Waterproof]

High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Document Storage Durable Metal Wire Mesh Decking for Pallet Rack, Part I.Mosquito Proof Fiberglass Fly Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Loader Xt860 Backhoe Loader with Digger]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board.19m Mobile LED Display Billboard for Advertising Screens, 175-184[Air Dryer Oil Lube Hydraulic Fuel Filter Auto Truck Parts for Komatsu B96]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Parts Stamping]

Whitaker,J.R.(1963),Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042). Analytical Chemistry35(12), 1950-1953[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with ValveAuthor for correspondence