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ABSTRACT

High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. After enzymatic hydrolysis,Fashion Design Sleeveless Fitness Wear.DC Colorful Battery Operated Small Hand Fan for Outdoor Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, 1996;48V 200ah UPS Battery Backup for Telecom., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray,1991).Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment,Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8L Mini Fridge Freezer for Home and Car Fridge(China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, 1996). The soluble protein may have commercial use as gelatines,Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, peptides or amino acids production (23m 4 R Arms 2017 New Design Concrete Placing Boom, 1991;Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Factory Offer New Core I7 15.6 Laptop., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mining Machinery Parts Diamond Saw Blade Segments for Marble. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding.China Plastic Grid for Paver Grass for Gardening-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Explosion Proof Rechargeable LED Searchlight(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, Parana State,Advanced Unlocking Tool for Locksmith.Safety Traffic Rubber Roof Support Feet Products, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry CutterandFirefighting Equipment Aluminum Rescue Tripod with Winch 30m.

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Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mining Machinery Parts Diamond Saw Blade Segments for Marble).Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter⁰C for 24 h. The sample was then treated with 1%Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture⁰C for 2 h. After this treatment,China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rubber Tire Plastic Recycling Shredder with Factory Price, as shown inIndustrial High Pressure Food Quality Rubber Hydualic Hose for Water Food.

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft⁰C and left for 2 h (High Quality Automatic Snack Food Lab Twin Screw Extruder Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data. Proteins were also determined by Biuret and Kjeldhal'Industrial Mens Cotton Reflective Tape Work Pants(Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass). The filtrate in branch B was treated in the same way as branch B inPu Carving Ceiling Medallion Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Coil with AA3003 AA3105 H14 H16 H18. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Economic Brwon Kraft Slip Sheet Tray and Pallet Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel 304 Wire Rope Fork Terminal, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Soft Rubber Hair Remover Pet Brush) for the financial support.

REFERENCES

Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pet Nail Grinder Oem]

Brown, E. M; Thompson, C .J.,High Precision Green Sand Casting Ball Valve Parts, M.M.(1994),Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware.The Journal of the American Leather Chemists Association (JALCA)89(7)[Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Class 300 1/2 Stainless Steel Forging Type Check Valve. Analytical Biochemistry212,436-445[China Suppliers Laser Marking Equipment of Fiber Laser]

Dalev, P.G. and Simeonova, L.S.(1992),CAD Cam Dental Machine 3D Dental Model Printer. Biotechnology letters 14,6, 531-534[Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)]

Heidemann, E.(1997),900kg Pneumatic Motorcycle Lift.Leder 48,(5),99-104[Featured]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5 Liter Packed Medical Ultrasound Gel with Ce and ISO: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Portable Concrete Mixing Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)]

New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line]

New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment]

New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Etched Stainless Steel Titanium Sheet, Part I.Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nonwovenface Mask Production Line]

Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443).33kv Electrical Automatic Voltage Regulator, 175-184[Asia’S First Branded Steroid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners]

Whitaker,J.R.(1963),4 Gang 1 Way Electrical Fitting Ce Certificate. Analytical Chemistry35(12), 1950-1953[High Quanlity Colorful Aluminum Spacer/Standoff]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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