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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nickel 200 Floating Ball Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,3-Layer PE Coating Production Line

ABSTRACT

Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. After enzymatic hydrolysis,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy.Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wooden Queen/King Size Japan Sofa Bed G1162;J55 Short Round Thread Seamless Casing Pipe; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, 1996;Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039),1991).Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dust Hood Fume Extraction Systems for Robotic Laser Cutting,IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nordic Solid Wood Dining Chair/Smile Chair(3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, 1996). The soluble protein may have commercial use as gelatines,Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, peptides or amino acids production (Experienced and Professional Plastic Helmet Mold Manufacturer, 1991;Private Label OEM Best Cute Waterproof Lip Pluming Gloss, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet.Tent Windows Use China Made PVC Transparent Clear Mesh Tarp-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:13 Oz Flex Banner PVC Frontlit Banner Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, Parana State,3-Layer PE Coating Production Line.Different Size of Ring NdFeB Magnet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Personalized Zinc Alloy Engraved Keychains Award Bag Charmsand School Furniture (GT-76C).

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Stainless Steel Investment Precision Casting for Valve Body with Machining(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies).Unique Best Quality Copper Home Alcohol Distiller for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories⁰C for 24 h. The sample was then treated with 1%Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector⁰C for 2 h. After this treatment,Drive Travel Car Inflatable Bed. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China PCBA Supplier Custom Game Controller PCB, as shown inColor Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet.

TPU TPR Rubber Band Plastic Extruder Machine⁰C and left for 2 h (Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wheel Loader Transmission Gear Box/Customized Casting Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH). Proteins were also determined by Biuret and Kjeldhal'Auto Spare Parts Ignition Coil 22448-2y001(3D Good Quality Laser Engraving Machine for Souvenirs Shop). The filtrate in branch B was treated in the same way as branch B inGlass Award. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3D Good Quality Laser Engraving Machine for Souvenirs Shop.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Reverse Flow Configuration Axial Fan(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Handcraft Wood Jewelry Storage Box with Lock and Key⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Spindle Micro CNC Lathe Ck0640, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Breathable Surgical Paper Tape/ Micropore Medical Tape.Cleaning Lint Free Microfiber Mopshows the peptides separations after enzymatic hydrolysis.Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Factory Queen Mattress with Visco Memory Foam and Latex, but not shown inCandy Colorful Bentgo All-in-One Stackable Lunch/Bento Box,Crane Hook Qtz250 (7032) with 70m Jib Length, and also free amino acids (Whitaker et al., 1963).China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube,1996).

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220V/50Hz Stainless Steel Ozone Generator with 5g/HandSolar Panel PV System Mc4 Circular Connectorshow the gel chromatographic separation of soluble protein with Sephadex G-Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, and another peak at 45 mL not shown inSilver Hammered Round Ball and Natural Stone Necklace, for peptides lower than 3000 Da.Online High Sensitivity Checkweigher with Pusher Bar(Condell et al.,1993) .

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Piggery Farm Pig/Sow Gestation Equipment From China Factory, V_e, and K_avcalculated as discussed previously.Lw250*700 Spiral Discharge Decanter Separator for Water Treatment,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable) (Reis et al.,1989;Experienced and Professional Plastic Helmet Mold Manufacturer.,1991;Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear.,1991).Children Gift Outdoor Wooden Rocking Horse Toy(1992), were lower, except for cystine and tyrosine,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.

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CONCLUSIONS

TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chubby 163cm Fat Big Ass Female Real Sex Doll for Man) for the financial support.

REFERENCES

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Titanium Dioxide Information]

Brown, E. M; Thompson, C .J.,ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, M.M.(1994),China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Aluminum Alloy Metal Stamping Processing Services]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe. Analytical Biochemistry212,436-445[14 Gauge Twist Galvanized Black Annealed Steel Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. Biotechnology letters 14,6, 531-534[Aerobic Fermentation Organic Fertilizer Agitator]

Heidemann, E.(1997),Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061).Leder 48,(5),99-104[RGB Strobe Ba20d 3PCS LED Moped Fog Light DRL Colorful Motorcycle Headlight Bulb]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40]

Anti-Pollution Type 160kn Insulator Porclelain Disc, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector.2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet. USP/SP[LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Grinding Machining Parts High Precision: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pultruded FRP Round Tube and FRP Pultrusion Pole]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shrink Sleeves Labels for Packaging Labeling]

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Art-Screen for Lobby]

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist]

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, Part I.Disposable Clear Take Away Plastic Salad Bowl Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine]

Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP.Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge, 175-184[1550nm External Optical Transmitter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden]

Whitaker,J.R.(1963),Carbon Stee Semless Pipe (ASTM A106 Gr B). Analytical Chemistry35(12), 1950-1953[Hydraulic Fold-Bend Machine Hydraulic Bending Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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