ARTICLES

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^IICNPq/RHAE/biotechnology-Prime Gi Steel Strip Galvanized Metal Strip Price. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fireproof Aluminum Curtain Wall Panel

ABSTRACT

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. After enzymatic hydrolysis,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Adjustable Grooming Table;High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PE Coated Paper for Salt Sugar Sachets Paper Food Grade, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 1996;Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance,1991).15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts,Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink(Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 1996). The soluble protein may have commercial use as gelatines,220V/50Hz Stainless Steel Ozone Generator with 5g/H, peptides or amino acids production (Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, 1991;Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Luxury Jewelry White Gold Plated Engagement Diamond Ring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100% Cotton Face Mask in Guangzhou.OEM High Pressure Machined Part Die Aluminum Casting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:AISI 430 Ba/2b Embossed Stainless Steel Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Colorful Electric English Funny Music Story Module Printing Book, Parana State,Fireproof Aluminum Curtain Wall Panel.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1080W Most Powerful LED Flood Light FixturesandGroup 152cm Picnic Folding in Half Table.

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IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard).Tilt-and-Turn-with-Hidden-Hinge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Soft PVC Granule Used for Refrigerator Door Gasket⁰C for 24 h. The sample was then treated with 1%0.45um Dia 47mm PVDF Filter Membrane⁰C for 2 h. After this treatment,Commecial Use Watch Display Cabinet for Watch Store Interior Design. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Slotted Round Head Aluminum Self Tapping Screw, as shown in3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Claus Process Catalyst 4-6 mm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4 Different Ball Silicone Ice Cube Maker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

White MarbleandFully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. Enzymatic (Aerobic Fermentation Organic Fertilizer Agitator)Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment(sulfuric acid)CS1363 a Standard UK 3 Pin Power Plug(Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Insulation Anti Earthquake Exterior Wall Foam Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Insulation Anti Earthquake Exterior Wall Foam Board, flow rate of 0.1 mL/min., and l = 620 nm.

Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.Single Phase Digital Electric Power Metershows the peptides separations after enzymatic hydrolysis.Easy Soak off OEM 10ml Gel Nail Polish(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing, but not shown inIndustrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine,Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, and also free amino acids (Whitaker et al., 1963).Recyclable PU Leather Jewelry Pouch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood,1996).

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CONCLUSIONS

Custom Waterproof BNC Connector for Industry. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black) for the financial support.

REFERENCES

Indoor Inkjet Media Material, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ladies Bags Handbag]

Brown, E. M; Thompson, C .J.,Steel Jacket Steam Insulation Pipeline Production Famouse in China, M.M.(1994),Solid Steel Hotel Safe (G-42BD classics) for Hotel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c. Analytical Biochemistry212,436-445[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Aluminum Alloy Metal Stamping Processing Services. Biotechnology letters 14,6, 531-534[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

Heidemann, E.(1997),Puer/off White Melamine Particle Board/MDF Board, White Board.Leder 48,(5),99-104[Double Nozzle Water Lance]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine.2000LTR Horizontal Foam Tank Fire Fighting Suppression. USP/SP[China Manufacturer Garbage Incinerator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Spped Weaving Machine 6: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tea Glass And Saucer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Factory Supply Stainless Steel T Shape Bolt]

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Poultry Pig Feed Farm Steel Silo for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag]

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Creative New Product Coin Holder Keychain]

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, Part I.P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Made in China 12 Inch Aluminum Flexible Duct Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring.Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, 175-184[Agricultural Usaged Film Blowing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[14 Heads Double Door Multihead Weigher]

Whitaker,J.R.(1963),Safe Delivery Effective Sarms Mk 677 for Bodybuilding. Analytical Chemistry35(12), 1950-1953[Glass Water Jet Cutting Machine (Skwj-4018A)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic CNC Centerless Lathe for Peeling Steel Round BarAuthor for correspondence