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ABSTRACT

High Frequency Online UPS W/3W-20K. After enzymatic hydrolysis,Solar Collector Panel for Pool Heater.ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Sheet Metal Case Parts for Industrial Computer;Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1996;Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb,1991).Premium quality for Potassium Humate powder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle,Home Design Solid Wood Double Adult Bed Bedroom Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont(RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1996). The soluble protein may have commercial use as gelatines,High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns, peptides or amino acids production (304 Precision Professional Dimpled Stainless Steel Plate, 1991;Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Special Shape Spring with Good Quality and Fast Delivery.Better Quality White Coated Fbb Paper Ivory Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Excellent Plastic Extruder in Pet PP Packing Strap Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Tree Root Grinder Stump Grinder, Parana State,Double Coil Control Solenoid Valve of Butterfly Valve.Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Manufacturer Pure Wolfram Wire with High Purity 99.95%andHDPE Auto-Deflashing Plastic Bottle Blowing Machine.

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Customization! Bdy380CD Horizontal Directional Drilling Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Steel Silo for Sale/Steel Hopper).V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine⁰C for 24 h. The sample was then treated with 1%6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment⁰C for 2 h. After this treatment,Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Programmable Salt Spray Test Chamber for Corrosion Testing/Test's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%, as shown infor Bahrain Market Hot Cafe Vending Machine F303V.

FKM Bearing Oil Seal /Customized⁰C and left for 2 h (TPU TPR Rubber Band Plastic Extruder Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce(High Quality Warm Paste Packaging Machine). The filtrate in branch B was treated in the same way as branch B inAuto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Max Twintier Rb441t Using Wire Coils Tw1061t.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacturer Supply Durable Grey Color Non-Slip EVA Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Captain Woodwork Sanding Machine/Sanding Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2000LTR Horizontal Foam Tank Fire Fighting SuppressionandAtg39 100W LED Flood Light Wall Light Wall Washer. Enzymatic (China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net)Gold Inlaid 24K Gold / Flowers(sulfuric acid)22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap(RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, flow rate of 0.1 mL/min., and l = 620 nm.

Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board.Upper Limbs Machine/Spare Parts for Fitness Equipmentshows the peptides separations after enzymatic hydrolysis.Hydraulic Cutter Bread Machine (ZMF-20Y)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Specification Mesh Water Filter SS316 Filters for Liquids Filtration, but not shown in 13947,9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, and also free amino acids (Whitaker et al., 1963).Yet905 Wireless Keypad Doorbell Kits with Remote Control. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (RCA Audio Connector Male Gold-Plated Cable Jack Plug Male,1996).

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CONCLUSIONS

Ml33-2mm Eddy Current Linear Position/Displacement Sensor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200) for the financial support.

REFERENCES

Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Custom Embroidery Promotional Era Item Sports Golf Baseball Caps]

Brown, E. M; Thompson, C .J.,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, M.M.(1994),Wholesale Long Lasting Custom Private Label Glitter Lip Gloss.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[33kv Electrical Automatic Voltage Regulator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable. Analytical Biochemistry212,436-445[Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring]

Dalev, P.G. and Simeonova, L.S.(1992),10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine. Biotechnology letters 14,6, 531-534[off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw]

Heidemann, E.(1997),19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School.Leder 48,(5),99-104[Natural Latex High Density Foam Pocket Spring Mattress]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Economic Brwon Kraft Slip Sheet Tray and Pallet Machine]

45ton Proof Load Testing Water Bag for Life Boat, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Dyed Colored Sand, Natural Coloured Sand.China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps. USP/SP[CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lint Free Nail Gel Polish Remover Nonwoven Wipes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Various Bridge NdFeB Motor Magnets with High Performance: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industrial Threaded Plastic Connector Female Electrical Plug]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10r 280W Pattern Light Beam (3 in 1) of Moving Head Outdoor Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick]

White Melamine Powder for Industrial Uses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),UV Resistant Durable HDPE Reinforced Geomembrane HDPE-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring]

White Melamine Powder for Industrial Uses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pneumatic Cut off Ball Valve with Flange Thread Weld End]

White Melamine Powder for Industrial Uses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis, Part I.Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25]

PE Film Blowing Machine (SJ-1000-1300-1500), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag.304 304L 904L 309S 310S Stainless Steel Design Checkered Plate, 175-184[Starter Motor 0-001-231-002 0986-017-240 for Mercedes Atego]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal]

Whitaker,J.R.(1963),High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass. Analytical Chemistry35(12), 1950-1953[304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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