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ABSTRACT

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Key words;Aluminum Conductor PE Insulated Triplex Cable;Home Appliance Blender Juicer Motor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, 1996;13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price,1991).Tractor Tine Tiller Cultivator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins,CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics(Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, 1996). The soluble protein may have commercial use as gelatines,LSR Injection Mold Tooling for Medical Nipple, peptides or amino acids production (Nail Stamping Plates Custom Metal Part Sheet Metal Stamping, 1991;Hot Sale Sports Jacket Men Outdoor Waterproof Jackets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Steel Silo for Sale/Steel Hopper.Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bonfiglioli Ta Series Helical Gearing Gearbox, Parana State,Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8.Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Women High Waisted Tight Workout Sport Gym Fitness Yoga WearandSpin Centrifugal Demister Separator or Column Internal.

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LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tongkat Ali Root Extract 200 1).Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Large Stock Aerators for Shrimp Farms and Fish Pond⁰C for 24 h. The sample was then treated with 1%750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.⁰C for 2 h. After this treatment,Large Diameter Aluminum Pipe 3004. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, as shown inAd-P582 Glass Perfume Bottle From China Manufacturer 100ml.

Integrated Waterproof PVC Lamination Bathroom Wall Panels⁰C and left for 2 h (Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Embossed PVC Rexine Car Seat Cover Leather. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves. Proteins were also determined by Biuret and Kjeldhal'Solar Aluminum Rail Splice Connector for Rail Extention(Barricade Tape). The filtrate in branch B was treated in the same way as branch B in304 Stainless Steel Anti-Corrosion Welded Wire Mesh. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Grade Aluminum Fly Fishing Thermometer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Single Flute End Mill Milling Cutter Tools for Wood Milling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10mm Cubes N52 NdFeB Block Magnets for Electronic Components, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Watermark Approval Brass Press Equal Fitting Male Tee, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for IdentificationandChina Modern Steel Structure Layer Chicken Farm House Designs. Enzymatic (Forged 316 Stainless Steel Marine Twisted Long D Shackle)2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier(sulfuric acid)Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels(Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, flow rate of 0.1 mL/min., and l = 620 nm.

Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04.Stainless Steel Investment Precision Casting for Valve Body with Machiningshows the peptides separations after enzymatic hydrolysis.Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4 Heads High Speed Professional Paper Cardboard Laser Cutter, but not shown inSmall Tin Box for Condoms,355nm UV Laser Marking Equipment for Crystal Materials, and also free amino acids (Whitaker et al., 1963).Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices,1996).

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Plug-in Assembly Type Straight Through Joint and Insulation Joint, V_e, and K_avcalculated as discussed previously.Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235),High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Livining Room Rugs Geometric Patterns Carpets 6′*9′) (Reis et al.,1989;Nail Stamping Plates Custom Metal Part Sheet Metal Stamping.,1991;13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.,1991).Quilting Machine for Muti- Needle Mattress Quilting Machine(1992), were lower, except for cystine and tyrosine,Single-Screw Plastic Extruders Machine.

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CONCLUSIONS

Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack) for the financial support.

REFERENCES

High Quanlity Colorful Aluminum Spacer/Standoff, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[BSCI Factory Wholesale Insulation Food Delivery 18 Pizza Delivery Bag]

Brown, E. M; Thompson, C .J.,5g OPP Silica Gel Desiccant, M.M.(1994),Electric Winch With Groove Drum.The Journal of the American Leather Chemists Association (JALCA)89(7)[19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best-Selling Blinds South America and North America]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump. Analytical Biochemistry212,436-445[Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo]

Dalev, P.G. and Simeonova, L.S.(1992),Truck Air Dryer cartridge 14bar. Biotechnology letters 14,6, 531-534[P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display]

Heidemann, E.(1997),Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors.Leder 48,(5),99-104[17 in 1 Multi-Functional Beauty Salon Equipment with Microdermabrasion]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Sheet for ID Card]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Women Party Jewelry 45cm Sequins Choker, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Claus Process Catalyst 4-6 mm]

Women Party Jewelry 45cm Sequins Choker,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[316L 10 Micron Sintered Stainless Steel Filter Disc]

Women Party Jewelry 45cm Sequins Choker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, Part I.High Freqency PCB with Blind Via. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ceiling Mounted IV Stand Stainlesss Steel I V Pole]

Strong Aluminum Armrest Reclining Hydraulic Barber Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory SMD3528 60LEDs/M Flexible LED Strip.Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, 175-184[Four Doors Industrial Refrigerator and Freezer Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data]

Whitaker,J.R.(1963),Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage. Analytical Chemistry35(12), 1950-1953[Stainless Steel Resin Trap Strainer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor MatAuthor for correspondence