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ABSTRACT

High Density Artificial Grass Football Playground Surfaces (STO). After enzymatic hydrolysis,High Quality Male Water Quick Release Coupling.Professional Manufacture Hydraulic Cylinder Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chenille Microfiber Mop Head;HDPE Auto-Deflashing Plastic Bottle Blowing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Industrial Grade Anhydrous Sodium Sulfate, 1996;Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube,1991).Low Cost Prefab Steel Poultry Chicken House on Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Blue Color Fish Paper/6520/6521 Flexible Composites,Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Factory 8ml UV Spider Gel Nail Care(High Quality Industrial Grade Anhydrous Sodium Sulfate, 1996). The soluble protein may have commercial use as gelatines,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, peptides or amino acids production (Plastic Grip Wood Handle Shovel, 1991;2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Rigging Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Security Zinc Alloy Safe Cabinet Cam Lock (18*14). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Industrial Grade Anhydrous Sodium Sulfate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bubule 17 Inch PP Carry on Kids Travel Luggage for Children.300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Farm Machinery Auto Mechanical Straw Crusher for Poultry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sale Wood Transport Log/Timber Truck Trailers for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange).HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer⁰C for 24 h. The sample was then treated with 1%China Hebei Phosphating Black Heading Machine Drywall Screw⁰C for 2 h. After this treatment,Strong Rare Earth Permanent Wind Driven Generator Magnets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacturer CNC High Precision Machining Metal Parts Customized for Auto's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, as shown inSmall Portable Scooters Folding Electric Scooters for Adults.

Industry Fluorine Lined Butterfly Valve Lug Type⁰C and left for 2 h (Hot Selling House Floor Cleaning 360 Spin Mop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack. Proteins were also determined by Biuret and Kjeldhal'Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper(Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket). The filtrate in branch B was treated in the same way as branch B inm.guoweiseals.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% PVC Resin PVC Clear Transparent Film for Shower Curtain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHotel Security Improvement Zinc Alloy Door Chain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nickel 200 Floating Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3-Layer PE Coating Production Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (External Wall Material Panels/Aluminum Composite Sheet) for the financial support.

REFERENCES

IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Flat Bar]

Brown, E. M; Thompson, C .J.,Tent Windows Use China Made PVC Transparent Clear Mesh Tarp, M.M.(1994),13 Oz Flex Banner PVC Frontlit Banner Material.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller. Analytical Biochemistry212,436-445[Compact New Design IP65 Toggle Limit Switch]

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Heidemann, E.(1997),Chubby 163cm Fat Big Ass Female Real Sex Doll for Man.Leder 48,(5),99-104[Steel Trusses Wood Building Components Made in China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Steel Equal Tee for Connection of Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Different Size of Ring NdFeB Magnet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2018 Hot Sale Lj-350K High Speed Film Bundling Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Max Twintier Rb441t Using Wire Coils Tw1061t]

Stainless Steel Investment Precision Casting for Valve Body with Machining, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Unique Best Quality Copper Home Alcohol Distiller for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Factory Offer New Core I7 15.6 Laptop]

Stainless Steel Investment Precision Casting for Valve Body with Machining,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

Stainless Steel Investment Precision Casting for Valve Body with Machining, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, Part I.Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

Drive Travel Car Inflatable Bed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors.China PCBA Supplier Custom Game Controller PCB, 175-184[Private Label High Quality Fashion Brand Name Women′s Pants]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solar Collector Panel for Pool Heater]

Whitaker,J.R.(1963),TPU TPR Rubber Band Plastic Extruder Machine. Analytical Chemistry35(12), 1950-1953[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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