ARTICLES

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ABSTRACT

Grade Aluminum Fly Fishing Thermometer. After enzymatic hydrolysis,CNC Single Flute End Mill Milling Cutter Tools for Wood Milling.Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sc Fiber Optic Fast Connector;Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10mm Cubes N52 NdFeB Block Magnets for Electronic Components, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Watermark Approval Brass Press Equal Fitting Male Tee, 1996;China Modern Steel Structure Layer Chicken Farm House Designs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Forged 316 Stainless Steel Marine Twisted Long D Shackle,1991).2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04(Watermark Approval Brass Press Equal Fitting Male Tee, 1996). The soluble protein may have commercial use as gelatines,Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, peptides or amino acids production (355nm UV Laser Marking Equipment for Crystal Materials, 1991;Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wire Mesh Welding Size 10mm X 10mm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.P4.75 Indoor Single Dual Color Advertising LED Display Billboard.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Watermark Approval Brass Press Equal Fitting Male Tee, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plug-in Assembly Type Straight Through Joint and Insulation Joint.Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

5g OPP Silica Gel Desiccant, Parana State,Solar Aluminum Rail Splice Connector for Rail Extention.Electric Winch With Groove Drum, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Watermark Approval Brass Press Equal Fitting Male Tee, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the120PCS/M Flexible LED Strip Tira De LED Light StripandGrade Aluminum Fly Fishing Thermometer.

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Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12 Teeth Green Painting Steel Rake).Truck Air Dryer cartridge 14bar(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors⁰C for 24 h. The sample was then treated with 1%3mm White Waterproof PVC Foam Board⁰C for 2 h. After this treatment,Educational Equipment Smart Multi Touch Interactive White Board for School. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Women Party Jewelry 45cm Sequins Choker, as shown inHOWO 420HP Rhd Tractor Truck Head.

New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity⁰C and left for 2 h (Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Freqency PCB with Blind Via. Proteins were also determined by Biuret and Kjeldhal'Strong Aluminum Armrest Reclining Hydraulic Barber Chair(New Style Custom Logo Makeup Multi Colored Eyeshadow Palette). The filtrate in branch B was treated in the same way as branch B in2 in 1 Mini Qi Fast Pad for Apple Watch 1 2 3 4 Series Airpods Wireless Charger. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory SMD3528 60LEDs/M Flexible LED Strip.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNarrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Comfort Furniture Natural Spring Memory Foam Mattress⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4.2A Type a Type C Pd QC3.0 USB Charger, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stc-001/Stc-100A/Stc/8080A+andFerrous Sulphate Monohydrate 30%Min Factory Wholesale. Enzymatic (Small Portable Scooters Folding Electric Scooters for Adults)Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant(sulfuric acid)DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet(Watermark Approval Brass Press Equal Fitting Male Tee, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Most Popular Split Pressurized Flat Panel Solar Water Heater.Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operationshows the peptides separations after enzymatic hydrolysis.Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files, but not shown inCe Approval Biscuit Trayless Packing Machine (MZ-250B),Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, and also free amino acids (Whitaker et al., 1963).Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Watermark Approval Brass Press Equal Fitting Male Tee,1996).

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RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, V_e, and K_avcalculated as discussed previously.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles,Cog Mono Round Display LCD Module. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player) (Reis et al.,1989;355nm UV Laser Marking Equipment for Crystal Materials.,1991;China Modern Steel Structure Layer Chicken Farm House Designs.,1991).4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black(1992), were lower, except for cystine and tyrosine,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric.

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CONCLUSIONS

China Products Suppliers Camlock Coupling Quick Couplings Female. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube) for the financial support.

REFERENCES

Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Benchtop Class B Autoclave Steam Sterilizer Laboratory Instrument Supplier in China]

Brown, E. M; Thompson, C .J.,3.2m Vertical Wheelchair Elevator Platform for Sale, M.M.(1994),LC Multistage Pump Mechanical Seal (KLLC).The Journal of the American Leather Chemists Association (JALCA)89(7)[Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Experienced and Professional Plastic Helmet Mold Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wedding Tent Cooling System Outdoor Event Air-Con Units. Analytical Biochemistry212,436-445[Petrochemical Loading Platform Folding Stairs]

Dalev, P.G. and Simeonova, L.S.(1992),M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV. Biotechnology letters 14,6, 531-534[Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack]

Heidemann, E.(1997),Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel.Leder 48,(5),99-104[Hot Products Sport Drawstring Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mooncake Pastry and Plastic Packaging Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Safe Delivery Effective Sarms Mk 677 for Bodybuilding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1/2 Inch Polyester Grosgrain Ribbon with Snow Printing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Density Insulation Rock Wool Board for Wall]

High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Adjustable Grooming Table, Part I.High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports]

PE Coated Paper for Salt Sugar Sachets Paper Food Grade, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood.Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, 175-184[100% Natural Plantain Ratio Extract]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hotbar Reflow Machine for FPC of Camera]

Whitaker,J.R.(1963),Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Analytical Chemistry35(12), 1950-1953[Food Grade Beverage Clear PVC Tubing Hose]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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