ARTICLES

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ABSTRACT

48V 200ah UPS Battery Backup for Telecom. After enzymatic hydrolysis,450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment;Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8L Mini Fridge Freezer for Home and Car Fridge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, 1996;23m 4 R Arms 2017 New Design Concrete Placing Boom., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle,1991).China Factory Offer New Core I7 15.6 Laptop. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates,Mining Machinery Parts Diamond Saw Blade Segments for Marble,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding(Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, 1996). The soluble protein may have commercial use as gelatines,China Plastic Grid for Paver Grass for Gardening, peptides or amino acids production (Explosion Proof Rechargeable LED Searchlight, 1991;Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Soft Rubber Hair Remover Pet Brush.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Forging Metal Building Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.Safety Traffic Rubber Roof Support Feet Products-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Jacket Steam Insulation Pipeline Production Famouse in China).Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data⁰C for 24 h. The sample was then treated with 1%Industrial Mens Cotton Reflective Tape Work Pants⁰C for 2 h. After this treatment,Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Coil with AA3003 AA3105 H14 H16 H18's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, as shown inFashion Water Drop Earring Charming 925 Silver Earrings.

DC Stand Fan Motor PCBA Card 24V⁰C and left for 2 h (Tie Down Nylon Back to Back Hook and Loop Straps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Economic Brwon Kraft Slip Sheet Tray and Pallet Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel 304 Wire Rope Fork Terminal. Proteins were also determined by Biuret and Kjeldhal'Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag(Animal Manure Fertilizer Fermentation Machine). The filtrate in branch B was treated in the same way as branch B inSUS/AISI (201/304) 2b Finished Cold/Hot Rolled Stainless Steel Strip/Coil Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Power Tools Lithium Battery Cordless Drill (GBK2-66108TS). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCAS 85-44 9 Flakes Phthali Anhydride(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Good Design Custom Made Cheap Recycle Paper Bags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Mini School Backpack for GirlsandNon-Ionic Polyacrylamide Flocculant. Enzymatic (Fashion Water Drop Earring Charming 925 Silver Earrings)Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS(sulfuric acid)Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks(Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Custom Power Extension Cord AC Cable Plug for Office Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture) for the financial support.

REFERENCES

Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[500ml Borosilicate Glass Water Bottle with Plastic Sleeve (CC-109)]

Brown, E. M; Thompson, C .J.,5 Liter Packed Medical Ultrasound Gel with Ce and ISO, M.M.(1994),New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre.The Journal of the American Leather Chemists Association (JALCA)89(7)[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Cheap Custom Festival Woven RFID Fabric Wristband for Events]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character. Analytical Biochemistry212,436-445[Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear]

Dalev, P.G. and Simeonova, L.S.(1992),Etched Stainless Steel Titanium Sheet. Biotechnology letters 14,6, 531-534[White Melamine Powder for Industrial Uses]

Heidemann, E.(1997),Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric.Leder 48,(5),99-104[3 Pin Delphi Pa66 Female Connector Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Air Duct Making Machine]

Outdoor Christmas Decoration Highway Street Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443). USP/SP[64mm Double Braid UHMWPE Tugboat Mooring Rope]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-33kv Electrical Automatic Voltage Regulator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Workshop Tools 7.5 Ton Electric Winch with Dual Speed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Products Needleless Injector Insulin Injection Sterilized]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue]

4 Gang 1 Way Electrical Fitting Ce Certificate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

4 Gang 1 Way Electrical Fitting Ce Certificate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tent Windows Use China Made PVC Transparent Clear Mesh Tarp]

4 Gang 1 Way Electrical Fitting Ce Certificate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6, Part I.PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Abrasive 6 Sanding Disc for Automotive Industry]

Rapid and Efficient Cooperation Multi Color Ribbon for Bows, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DMD Polyester Film/6630/Electric Insulation Paper.Customized Hardware Aluminum Alloy Die Casting, 175-184[Y32-200 Ton Wheelbarrow Edge Cutting and Curling Hydraulic Press Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Peptides Powder Ghrp 2 for Muscle Building with Super Quality]

Whitaker,J.R.(1963),Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles. Analytical Chemistry35(12), 1950-1953[Hot Sale Electrical Motors Small for Home Appliance]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor PressAuthor for correspondence