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ABSTRACT

Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. After enzymatic hydrolysis,Car Trunk Organizer Car Storage Organizer.Fashion Cotton Baby/Kid Hat (DH-BF332). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line;OEM Round Stainless Steel Fruit Plate Tray; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Industry Grade Sodium Gluconate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, 1996;En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini High Temperature Clothing Labels Screen Printing Machine Factory,1991).Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale,Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Conventional Best-Selling Household Bamboo Craft Chopsticks(Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, 1996). The soluble protein may have commercial use as gelatines,High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, peptides or amino acids production (Ce Marked Medical Syringe Pump (WP08N), 1991;New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electro Galvanized Low Carbon Straight Cut Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Geotextile for Roofs, Civil Applications. Railway Work.Pneumatic Shuttle Valve Flange Ends-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stx825 Dual 15 Powerful PA Speaker, Speaker Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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5 Gallon Pure Water Bottle Non-Spill Cap⁰C and left for 2 h (40W Integrated Solar Street Lighting LED for Park). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. Proteins were also determined by Biuret and Kjeldhal'Solid Carbide Single Flute End Mills Oblique Angle Cutting(FKM Rubber Gasket/Rubber O Rings in China). The filtrate in branch B was treated in the same way as branch B in1500rpm Dc Brushless Motor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Seasoned Cast Iron South Africa Three Legged Potjie Pot⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Aluminum Carpet Topping Stage Platform. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible) for the financial support.

REFERENCES

BY60100C type metal shaping machine/hydraulic shaper machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Essential Oil Diffuser]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DC Colorful Battery Operated Small Hand Fan for Outdoor Use]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud. Analytical Biochemistry212,436-445[Factory Supply Industry Grade Sodium Gluconate]

Dalev, P.G. and Simeonova, L.S.(1992),Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard. Biotechnology letters 14,6, 531-534[Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lw250*700 Spiral Discharge Decanter Separator for Water Treatment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Line Filter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Different Dimension Rectangular Flexible Waveguide]

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Masturbators Adult Sex Toys Japanese Love Doll,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Super Potassium Humate Shiny Flakes]

Masturbators Adult Sex Toys Japanese Love Doll, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carbon Steel Spherical Plain Radical Bearings, Part I.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

Whitaker,J.R.(1963),Small Tin Box for Condoms. Analytical Chemistry35(12), 1950-1953[HVAC Ventilation Supply Air Return Grille Air Vent]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335cAuthor for correspondence