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ABSTRACT

110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator. After enzymatic hydrolysis,Supply 20-110mm PP Reducing Tee for Pipe Systems.5 Gallon Pure Water Bottle Non-Spill Cap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks;7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solid Carbide Single Flute End Mills Oblique Angle Cutting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies, 1996;ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films,1991).Small Capacity Automatic Cup Filling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Seasoned Cast Iron South Africa Three Legged Potjie Pot,Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine(High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies, 1996). The soluble protein may have commercial use as gelatines,2019 New Release Doypack Sachet Powder Packing Machine Price, peptides or amino acids production (Amplifier Tube Glass Vacuum Tube (833C), 1991;Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Style Custom Logo Makeup Multi Colored Eyeshadow Palette., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu.High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Daytai 1550nm External Modulation Optical Transmitter 1550 -50km(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Construction Scaffolding Steel Galvanized Acrow Prop).Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Aluminum Carpet Topping Stage Platform⁰C for 24 h. The sample was then treated with 1%BY60100C type metal shaping machine/hydraulic shaper machine⁰C for 2 h. After this treatment,Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Spplier 70W Solar Street Light with Camera Monitoring System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, as shown inFlat Pressure Indentation Punching Machine Automatic Card Box Die Cutting.

Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard⁰C and left for 2 h (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader. Proteins were also determined by Biuret and Kjeldhal'Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush(Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance). The filtrate in branch B was treated in the same way as branch B in30ml Round Shape White Pet Dropper Bottles with Matte Silver Cap White Rubber Dropper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Masturbators Adult Sex Toys Japanese Love Doll. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Rubber Soles Vulcanizing Machine/ Press Machine.Online High Sensitivity Checkweigher with Pusher Barshows the peptides separations after enzymatic hydrolysis.Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQg Jg Fg Series Air Dryer Equipment Factory, but not shown inHigh Quality Bucket Segment Bolt 6V0937,Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032, and also free amino acids (Whitaker et al., 1963).AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies,1996).

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Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, V_e, and K_avcalculated as discussed previously.CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping,Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Children Gift Outdoor Wooden Rocking Horse Toy) (Reis et al.,1989;Amplifier Tube Glass Vacuum Tube (833C).,1991;ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat.,1991).Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)(1992), were lower, except for cystine and tyrosine,2018 New Design Red-CNC-006 CNC Machining Aluminum Precision.

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CONCLUSIONS

China Factory Price Hg1 Self Adjustable Pipe Tank Rotator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit) for the financial support.

REFERENCES

High Quality Crawler Excavator Track Bolt 3p2274, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Gold Visor Hat]

Brown, E. M; Thompson, C .J.,SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, M.M.(1994),Automatic CNC Centerless Lathe for Peeling Steel Round Bar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polypropylene Fibre for Commercial Slabs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. Analytical Biochemistry212,436-445[ Lotion Noodle Jam]

Dalev, P.G. and Simeonova, L.S.(1992),Ck6150 High Precision Metal Machining Horizontal CNC Lathe. Biotechnology letters 14,6, 531-534[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly]

Heidemann, E.(1997),Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting.Leder 48,(5),99-104[UV Laser Marking Machine for PP PE Plastic Glass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flat Roof Insulation Board Heat Insulation Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Di003 Environmental Protection Against The Stench Floor Drain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Custom Round Aluminum Tube 5154 5454 5652 5086: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LSR Injection Mold Tooling for Medical Nipple]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Promotion Standard Hospital Plastic Clipboard]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5km City Surveillance Network Infrared Laser PTZ Camera]

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Newest Adjustable Decline Bench Flat Weight Bench for Wholesales,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Current Precise LED Digital Ammeter]

Newest Adjustable Decline Bench Flat Weight Bench for Wholesales, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, Part I.LED UV Portable Toothbrush Sterilizer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter]

Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),American NEMA Straight Blade Plug 30A 125V/250V Power Plug.10in 1500lb Pressure Seal Forged Steel Swing Check Valve, 175-184[2018 Facial Therapy 7 Color Photon LED Skin Rejuvenation]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle]

Whitaker,J.R.(1963),Custom Printed/Embossed Black Earring Packaging Cards. Analytical Chemistry35(12), 1950-1953[Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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