ARTICLES

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ABSTRACT

Rapid and Efficient Cooperation Multi Color Ribbon for Bows. After enzymatic hydrolysis,DMD Polyester Film/6630/Electric Insulation Paper.Customized Hardware Aluminum Alloy Die Casting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, 1996;Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation,1991).5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,600mm Warehouse No Power Roof Fan,Small Brown Glass Bottles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag(Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, 1996). The soluble protein may have commercial use as gelatines,Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe, peptides or amino acids production (RF China Professional Manufacturer Direct Way Manual Ball Valve, 1991;Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Braided PTFE Hose R14 Standard Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Black Steel ASTM A192 Seamless Pipe Sch120. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Raw Material Adhesive NBR Latex of Latex Long Gloves.Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cracking Acidification Clay Stabilizer Pale Yellow Liquid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling).High Tensisle Strength Tissue Paper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Zinc Coated Galvanized Wire⁰C for 24 h. The sample was then treated with 1%Women Summer Swimsuit Chest Zip Bathing Suit⁰C for 2 h. After this treatment,Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Female Header Conn Ptich 1.0mm Connector Btb's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tie Down Nylon Back to Back Hook and Loop Straps, as shown inIntegrated Waterproof PVC Lamination Bathroom Wall Panels.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Resin Trap Strainer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Bar Fridge with Glass Door for Beverage and Beer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap Loader Xt860 Backhoe Loader with Digger⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fast Install Polyurethane Decorative Wall Panel for Homes) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather, M.M.(1994),Cleaning Lint Free Microfiber Mop.The Journal of the American Leather Chemists Association (JALCA)89(7)[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Making Machine for EVA Soft Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm. Analytical Biochemistry212,436-445[Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Crawler Excavator Track Bolt 3p2274]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Australian/Us/EU Standard Aluminium/Aluminum Sliding Door and Window]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory SMD3528 60LEDs/M Flexible LED Strip]

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold]

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Color Coated Corrugated Galvanized Roofing Steel Plate]

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cjx2-F (LC1-F) AC Magnetic Contactor, Part I.30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine]

Whitaker,J.R.(1963),Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11. Analytical Chemistry35(12), 1950-1953[Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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