ARTICLES

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ABSTRACT

Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. After enzymatic hydrolysis,Women′s Seamless Strapless Bra.Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm;Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, 1996;Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting,1991).Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal,20 Experiences on Plastic Injection Moulding Service,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wind Protection Screen. Wind Dust Netting, Wind Dust Net(off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, 1996). The soluble protein may have commercial use as gelatines,24K Gold Rolling Paper Shine Papers Cigarette Paper, peptides or amino acids production (Two - Way Double Row Thrust Tapered Roller Bearings, 1991;Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ride on Toys Riding Animals in The Mall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Finely Processed Pneumatic Operated Globe Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Color Coated Corrugated Galvanized Roofing Steel Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Precision Diamond Wire Saw Rope for Cutting Concrete Stone(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Workbench Anti-Static Table ESD Natural Rubber Table Mat⁰C and left for 2 h (Wholesale Mini School Backpack for Girls). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.316L 10 Micron Sintered Stainless Steel Filter Disc. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. Proteins were also determined by Biuret and Kjeldhal'Abstract Light and Shadow Metal Oil Painting for Deco(Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer). The filtrate in branch B was treated in the same way as branch B inHigh Quality 3d Chrome Metal Letter Signs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheapest Container Living House/Office/Kiosk. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSpin Centrifugal Demister Separator or Column Internal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/360 Degree Reciprocating Car Wash Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Glass Disc Suspension Insulators. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring) for the financial support.

REFERENCES

2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Indoor Decoration PVC Panel/PVC Interior Decorative Wall Panels/PVC Ceiling]

Brown, E. M; Thompson, C .J.,Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.M.(1994),Polypropylene Fibre for Commercial Slabs.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[White Melamine Powder for Industrial Uses]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. Analytical Biochemistry212,436-445[Air Duct Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Glass Water Jet Cutting Machine (Skwj-4018A). Biotechnology letters 14,6, 531-534[64mm Double Braid UHMWPE Tugboat Mooring Rope]

Heidemann, E.(1997),U. S. Type Clevis Grab Hook.Leder 48,(5),99-104[magnet bar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment]

Green PVC Coated Front Yard Double Wire Mesh Fence Panel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale.Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. USP/SP[Workshop Tools 7.5 Ton Electric Winch with Dual Speed]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Car Trunk Organizer Car Storage Organizer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Engineering Plastic Handle Bridge Handles Center Hole 90mm]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tent Windows Use China Made PVC Transparent Clear Mesh Tarp]

Fashion Cotton Baby/Kid Hat (DH-BF332), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Abrasive 6 Sanding Disc for Automotive Industry]

Fashion Cotton Baby/Kid Hat (DH-BF332),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Peptides Powder Ghrp 2 for Muscle Building with Super Quality]

Fashion Cotton Baby/Kid Hat (DH-BF332), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Round Stainless Steel Fruit Plate Tray, Part I.Factory Supply Industry Grade Sodium Gluconate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Electrical Motors Small for Home Appliance]

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube.Mini High Temperature Clothing Labels Screen Printing Machine Factory, 175-184[Ozone For Drinking Water Treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press]

Whitaker,J.R.(1963),Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. Analytical Chemistry35(12), 1950-1953[Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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