ARTICLES

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ABSTRACT

Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. After enzymatic hydrolysis,Linear Vibrator Screener, for Granule and Powder Selection and Classification.Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Children Hardcover Book Printing Services with Free Printing File Checking;One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, 1996;Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt,1991).25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Size Stone Aluminum Honeycomb Panel for Facade,Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging(New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, 1996). The soluble protein may have commercial use as gelatines,Making Machine for EVA Soft Hose, peptides or amino acids production (Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474, 1991;High Efficiency Air Cooled Air Source Water Chiller Heat Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12oz 16oz Glass Mason Jar with Handle and Straw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mini Freeze Dryer for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber.Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, Parana State,LED Fairy Star Light for All Kinds of Decoration Skylike.0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePrivate Label OEM Best Cute Waterproof Lip Pluming GlossandMore Cavities Plastic Mould Design.

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Weiying Full Computerized Plain and Invisible Sock knitting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Tensisle Strength Tissue Paper).Hot Double Cereal Airtight Food Dispenser(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Disposable Food Packaging Paper Soup Bowl with Lids⁰C for 24 h. The sample was then treated with 1%500ml PP Sport Water Bottle with Ball⁰C for 2 h. After this treatment,Stamping Fine Blanking Sheet Metal Fabrication Part. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Trade Assurance Beautiful Silk Lace Closure, as shown inChinese Color Steel Glazed Tile Purlin Roll Forming Machine.

High Quality Needle Rollers for Bearings⁰C and left for 2 h (150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heavy Duty Steel Warehouse Storage Pallet Rack. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab. Proteins were also determined by Biuret and Kjeldhal'High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire(Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength). The filtrate in branch B was treated in the same way as branch B inTransparent Clear UV Coating Solid Polycarbonate Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Bulk Price Yk-11 Powder for Bodybuilding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.380V 3.0HP Peristaltic Pump Swimming Pool Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/14 Gauge Twist Galvanized Black Annealed Steel Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrestand2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. Enzymatic (Fully Automatic Digital Pid Temperature Controller)High Quality Anti-Corrosion Fiberglass Sheet and Roll(sulfuric acid)Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter(New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Full-Automatic Folding Napkin Paper Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Full-Automatic Folding Napkin Paper Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Customized Frame Mount Highway Pegs Enclosure Parts.Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Partsshows the peptides separations after enzymatic hydrolysis.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBallscrew Fixed Support Unit Fk12 with High Quality for The Module, but not shown inRectangular Lunch Tin Box with Handle,10mm Tempered Bathroom Glass Safety Glass for Building, and also free amino acids (Whitaker et al., 1963).3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw,1996).

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25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for ZimbabweandWeak Daylight Recharge All in One Solar Street Lightshow the gel chromatographic separation of soluble protein with Sephadex G-Cattle Feed Factory Used Dust Collector and Filter.Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, and another peak at 45 mL not shown in SBPP, for peptides lower than 3000 Da.Group 152cm Picnic Folding in Half Table(Condell et al.,1993) .

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Tactile Custom Branded Membrane Switch Keyboard with Foil, V_e, and K_avcalculated as discussed previously.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm,Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Inclined Waste Water Treatment Lamella Clarifier) (Reis et al.,1989;Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474.,1991;Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette.,1991).Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags(1992), were lower, except for cystine and tyrosine,Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.

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CONCLUSIONS

Australia Two Pins Power Cord with SAA Certification. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flat Head Six-Lobe with Gasket Self Tapping Screws) for the financial support.

REFERENCES

87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4 Liter Hotel Mini Fridge]

Brown, E. M; Thompson, C .J.,Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, M.M.(1994),Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cvkr Silent Check Valve Dn350]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Crane Hook Qtz250 (7032) with 70m Jib Length]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. Analytical Biochemistry212,436-445[120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay]

Dalev, P.G. and Simeonova, L.S.(1992),Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. Biotechnology letters 14,6, 531-534[Men Casual Short Pants Custom Quality Running Sports Wear Shorts]

Heidemann, E.(1997),Pickled Cucumber Production Line/Making Machine.Leder 48,(5),99-104[New Dersign Outdoor Playground Amusement Equipment (QL--055)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course]

Lf + Hf Contactless Smart Hybrid Card, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rectangular Lunch Tin Box with Handle.Inflatable Cheering Stick for Promotional Gift. USP/SP[ Injection Molding]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12/24V 150ah Power Battery Charger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Hex Self Drilling Tapping Screw Tek Screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Women Summer Swimsuit Chest Zip Bathing Suit]

Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Finely Processed Pneumatic Operated Globe Valve]

Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, Part I.2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Precision Green Sand Casting Ball Valve Parts]

Mini Outdoor Hydraulic Man Aluminum Lift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap Price Roof Wall PU Sandwich Panel Press.Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, 175-184[0 Mg Nicotine 30 Ml  and Tobacco Free E Liquid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Spped Weaving Machine 6]

Whitaker,J.R.(1963),industrial aluminum foil roll 8011 8079 1235. Analytical Chemistry35(12), 1950-1953[Car Parking or Access Control 2.4 GHz Active RFID Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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