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ABSTRACT

Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine. After enzymatic hydrolysis,Large Stock Aerators for Shrimp Farms and Fish Pond.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Large Diameter Aluminum Pipe 3004;Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Integrated Waterproof PVC Lamination Bathroom Wall Panels, 1996;Embossed PVC Rexine Car Seat Cover Leather., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves,1991).Solar Aluminum Rail Splice Connector for Rail Extention. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Grade Aluminum Fly Fishing Thermometer,CNC Single Flute End Mill Milling Cutter Tools for Wood Milling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light(Integrated Waterproof PVC Lamination Bathroom Wall Panels, 1996). The soluble protein may have commercial use as gelatines,Sc Fiber Optic Fast Connector, peptides or amino acids production (Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, 1991;10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Watermark Approval Brass Press Equal Fitting Male Tee., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Modern Steel Structure Layer Chicken Farm House Designs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Forged 316 Stainless Steel Marine Twisted Long D Shackle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Integrated Waterproof PVC Lamination Bathroom Wall Panels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier.Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wire Mesh Welding Size 10mm X 10mm, Parana State,LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L.P4.75 Indoor Single Dual Color Advertising LED Display Billboard, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Integrated Waterproof PVC Lamination Bathroom Wall Panels, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCarbon Steel Round/Square Pipe Tube Production Lineand1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector.

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New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FKM Bearing Oil Seal /Customized).Plug-in Assembly Type Straight Through Joint and Insulation Joint(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)⁰C for 24 h. The sample was then treated with 1%High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin⁰C for 2 h. After this treatment,Quilting Machine for Muti- Needle Mattress Quilting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Single-Screw Plastic Extruders Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, as shown in044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head.

High Quanlity Colorful Aluminum Spacer/Standoff⁰C and left for 2 h (Vacuum Type Single Facer Corrugator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5g OPP Silica Gel Desiccant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Winch With Groove Drum. Proteins were also determined by Biuret and Kjeldhal'Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump(Stainless Steel Handweel Three-Way Non-Retention Ball Valve). The filtrate in branch B was treated in the same way as branch B inDie Punching Parts Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Truck Air Dryer cartridge 14bar.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3mm White Waterproof PVC Foam Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Women Party Jewelry 45cm Sequins Choker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work GloveandHigh Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. Enzymatic (High Freqency PCB with Blind Via)Strong Aluminum Armrest Reclining Hydraulic Barber Chair(sulfuric acid)Factory SMD3528 60LEDs/M Flexible LED Strip(Integrated Waterproof PVC Lamination Bathroom Wall Panels, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, flow rate of 0.1 mL/min., and l = 620 nm.

Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.Pengkai Domestic Underground Sewage Treatment Plant Aao Processshows the peptides separations after enzymatic hydrolysis.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHardware Rigging Forged Red Weldless Pear Shaped Link, but not shown inShs250X500 Galvanized Square Rectangular Steel Tube Price,Comfort Furniture Natural Spring Memory Foam Mattress, and also free amino acids (Whitaker et al., 1963).Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Integrated Waterproof PVC Lamination Bathroom Wall Panels,1996).

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Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, V_e, and K_avcalculated as discussed previously.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet,Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck) (Reis et al.,1989;Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue.,1991;Embossed PVC Rexine Car Seat Cover Leather.,1991).Most Popular Split Pressurized Flat Panel Solar Water Heater(1992), were lower, except for cystine and tyrosine,Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter.

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CONCLUSIONS

Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (355nm UV Laser Marking Equipment for Crystal Materials) for the financial support.

REFERENCES

Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Products]

Brown, E. M; Thompson, C .J.,Customized UV Drying Heating Machine for Screen Printer, M.M.(1994),99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide).The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Jacket Steam Insulation Pipeline Production Famouse in China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion Water Drop Earring Charming 925 Silver Earrings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947. Analytical Biochemistry212,436-445[Tie Down Nylon Back to Back Hook and Loop Straps]

Dalev, P.G. and Simeonova, L.S.(1992),RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. Biotechnology letters 14,6, 531-534[Animal Manure Fertilizer Fermentation Machine]

Heidemann, E.(1997),Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.Leder 48,(5),99-104[Cages For Breeders Hens]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Mini School Backpack for Girls]

Bonfiglioli Ta Series Helical Gearing Gearbox, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cog Mono Round Display LCD Module.4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. USP/SP[Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum/Aluminium Extrusion Profile Housing for LED Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beech]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles]

China Products Suppliers Camlock Coupling Quick Couplings Female, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Female Threaded Tee T Shape Pipe Fittings]

China Products Suppliers Camlock Coupling Quick Couplings Female,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics]

China Products Suppliers Camlock Coupling Quick Couplings Female, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3.2m Vertical Wheelchair Elevator Platform for Sale, Part I.LC Multistage Pump Mechanical Seal (KLLC). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale]

Wedding Tent Cooling System Outdoor Event Air-Con Units, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, 175-184[Tungsten Alloy Shot]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Iron Wire Dry Type Straight Line Wire Drawing Machine]

Whitaker,J.R.(1963),Prime Gi Steel Strip Galvanized Metal Strip Price. Analytical Chemistry35(12), 1950-1953[Oral Thermometer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube WallAuthor for correspondence