ARTICLES

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ABSTRACT

5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve. After enzymatic hydrolysis,600mm Warehouse No Power Roof Fan.Small Brown Glass Bottles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.RF China Professional Manufacturer Direct Way Manual Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, 1996;Stainless Braided PTFE Hose R14 Standard Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser,1991).Black Steel ASTM A192 Seamless Pipe Sch120. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Raw Material Adhesive NBR Latex of Latex Long Gloves,Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw(Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, 1996). The soluble protein may have commercial use as gelatines,Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress, peptides or amino acids production (1200X1000X810 Strong Collapsible Plastic Containers for Storage, 1991;Fast Install Polyurethane Decorative Wall Panel for Homes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Factory Supply Stainless Steel T Shape Bolt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cracking Acidification Clay Stabilizer Pale Yellow Liquid.High Tensisle Strength Tissue Paper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Zinc Coated Galvanized Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, Parana State,50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed SwitchandAuto Parts Durable Iridium Spark Plug OEM 5303 Ik16.

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Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Professional Shipping Agent to Turkey).Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Warm Paste Packaging Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel Resin Trap Strainer⁰C for 2 h. After this treatment,Small Bar Fridge with Glass Door for Beverage and Beer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cheap Loader Xt860 Backhoe Loader with Digger, as shown inFireproof Aluminum Curtain Wall Panel.

2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle⁰C and left for 2 h (CAS 85-44 9 Flakes Phthali Anhydride). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lithium Battery 20ah Pouch 3c Cell 3.7V. Proteins were also determined by Biuret and Kjeldhal'Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk(Fashion Cotton Baby/Kid Hat (DH-BF332)). The filtrate in branch B was treated in the same way as branch B inMedical Device Metal Keyboard. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Handweel Three-Way Non-Retention Ball Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Factory Supply Industrial Machine Pasta. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUltra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.for Bahrain Market Hot Cafe Vending Machine F303V⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Teflon for Industrial Plastic Gasket Seal with Good QualityandCNC Glass Cutting Line. Enzymatic (Small & Tiny CNC Precision Machining Brass Parts Metal Part)Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(sulfuric acid)2019 Kiet Hydraulic Pipe Bender Machine(Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine.Quilting Machine for Muti- Needle Mattress Quilting Machineshows the peptides separations after enzymatic hydrolysis.LED UFO Highbay 250W, 22500 Lumens(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, but not shown inSand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating,Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, and also free amino acids (Whitaker et al., 1963).Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected,1996).

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CONCLUSIONS

Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Female Header Conn Ptich 1.0mm Connector Btb) for the financial support.

REFERENCES

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[D]

Brown, E. M; Thompson, C .J.,Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, M.M.(1994),Cjx2-F (LC1-F) AC Magnetic Contactor.The Journal of the American Leather Chemists Association (JALCA)89(7)[19m Mobile LED Display Billboard for Advertising Screens]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal. Analytical Biochemistry212,436-445[10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting]

Dalev, P.G. and Simeonova, L.S.(1992),Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH). Biotechnology letters 14,6, 531-534[AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe]

Heidemann, E.(1997),Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan.Leder 48,(5),99-104[Wholesale Luggage Straps with Tsa Locks]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lf + Hf Contactless Smart Hybrid Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Mode Sc/APC Simplex Fiber Optic Adapter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electro Galvanized Steel Wire/Q195/Q235/Building Material Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Small Portable Scooters Folding Electric Scooters for Adults]

120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Selling House Floor Cleaning 360 Spin Mop]

120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket]

120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Abrasive 6 Sanding Disc for Automotive Industry, Part I.Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube]

2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm.Single Sphere Rubber Expansion Joint, 175-184[Motorcycle Accessory Speedometer Cable for Honda Cg150titan Ks/Es 2004/2008]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket]

Whitaker,J.R.(1963),Cold Press Circular Wood Cutting Machine Blade. Analytical Chemistry35(12), 1950-1953[Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tree Root Grinder Stump GrinderAuthor for correspondence