ARTICLES

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ABSTRACT

European Hotel Decorative Natual Shell Square Standing Floor Lamp. After enzymatic hydrolysis,Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT.Pengkai Domestic Underground Sewage Treatment Plant Aao Process. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solar DC Water Pump, Solar Submersible Pumping System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, 1996;Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V,1991).China Manufacturer Mini Cultivator for Small or Garden Farming. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light,Stainless Steel Art-Screen for Lobby,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch(55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, 1996). The soluble protein may have commercial use as gelatines,Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women, peptides or amino acids production (Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat, 1991;Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Samples Free! ! ! Primary/Remelted/Refined Lead Ingots.Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:120PCS/M Flexible LED Strip Tira De LED Light Strip(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, Parana State,IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera.High Quality Sesame Bean Seed Cleaner and Grader, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding MachineandVenlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden.

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Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Slotted Round Head Aluminum Self Tapping Screw).Pet Strapping Band Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label⁰C for 24 h. The sample was then treated with 1%Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration⁰C for 2 h. After this treatment,PA Flexible Plastic Corrugated Loom Tubing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, as shown in24K Gold Rolling Paper Shine Papers Cigarette Paper.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTwisted Annealed Wire for Binding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Fashion Jewellery Lady Eardrop Earrings for Women⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mini Freeze Dryer for SaleandFull Automatic AAC Concrete Lightweight Block Making Machine. Enzymatic (Stainless Steel CF8/CF8m Street Elbow Pipe Fittings)Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding(sulfuric acid)China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service(55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, flow rate of 0.1 mL/min., and l = 620 nm.

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Hot Sale Sports Jacket Men Outdoor Waterproof JacketsandFactory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Boardshow the gel chromatographic separation of soluble protein with Sephadex G-Carbon Steel Round/Square Pipe Tube Production Line.Green PVC Coated Front Yard Double Wire Mesh Fence Panel, and another peak at 45 mL not shown inWholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, for peptides lower than 3000 Da.Cable Length 11 Gun Safety Padlock(Condell et al.,1993) .

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Canvas School College Backpack/Bookbags for Girls/Students/Women, V_e, and K_avcalculated as discussed previously.Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles,Women′s Seamless Strapless Bra. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector) (Reis et al.,1989;Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat.,1991;Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow.,1991).Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet(1992), were lower, except for cystine and tyrosine,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm.

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CONCLUSIONS

Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage) for the financial support.

REFERENCES

(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic flakes granule vertical centrifugal dewatering machine]

Brown, E. M; Thompson, C .J.,off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, M.M.(1994),Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting. Analytical Biochemistry212,436-445[Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering]

Dalev, P.G. and Simeonova, L.S.(1992),Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Biotechnology letters 14,6, 531-534[Galvanized Steel Grating for Metallurgy]

Heidemann, E.(1997),Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal.Leder 48,(5),99-104[m.meditecho2.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel 304 Wire Rope Fork Terminal]

Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),20 Experiences on Plastic Injection Moulding Service.Wind Protection Screen. Wind Dust Netting, Wind Dust Net. USP/SP[Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Outdoor Hydraulic Man Aluminum Lift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-24K Gold Rolling Paper Shine Papers Cigarette Paper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[50l Electric Glass Lined Reactor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bathroom Makeup LED Mirror Glass with Light]

Two - Way Double Row Thrust Tapered Roller Bearings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Canvas School College Backpack/Bookbags for Girls/Students/Women]

Two - Way Double Row Thrust Tapered Roller Bearings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality Custom Car Power Window Cable for Ford Car]

Two - Way Double Row Thrust Tapered Roller Bearings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ride on Toys Riding Animals in The Mall, Part I.Finely Processed Pneumatic Operated Globe Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment]

Color Coated Corrugated Galvanized Roofing Steel Plate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, 175-184[Antminer Supplier & Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Dyed Colored Sand]

Whitaker,J.R.(1963),Precision Diamond Wire Saw Rope for Cutting Concrete Stone. Analytical Chemistry35(12), 1950-1953[Forging Metal Building Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber CableAuthor for correspondence