ARTICLES

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ABSTRACT

Compression Mens Customized Gym Shorts Sportswear. After enzymatic hydrolysis,Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip.99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder;P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Furniture Lift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, 1996;Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Construction Material Hoist Rack and Pinion,1991).3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks,Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses(Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, 1996). The soluble protein may have commercial use as gelatines,High Quality Endoscopic Surgical Adult Disposable Bite Block, peptides or amino acids production (China OEM ABS Clear Plastic Injection Molding, 1991;Widely Use 32 Cavities Pet Preform Water Bottle Mould, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Powder Coated Steel Sheet Metal Housing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sell 100% Seal Hexagon Glass Jam Jar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping.High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Heavy Caliber Copper Rectangular Steel TubeAlcalase2.5 L DX,2mm White Coated Paper Cake Boards Jordan, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Luxious Hotel Passenger Elevator, Titanium Elevator(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Yuchai 400kw Diesel Generator Set, Parana State,Carbide Mould Parts Moulding Parts.Black Plastic GRP FRP PE Pipe Fitting for Water Supply, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAir Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting MachineandMini High Temperature Clothing Labels Screen Printing Machine Factory.

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2194230012 for Mercedes Benz Auto Spare Parts Brake Disc(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Workbench Anti-Static Table ESD Natural Rubber Table Mat).Precision Casting Factory Foundry OEM Ductile Iron Sand Casting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Electrical Motors Small for Home Appliance⁰C for 24 h. The sample was then treated with 1%PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line⁰C for 2 h. After this treatment,Bending Shrapnel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V Car Cigarette Lighter Adapter Power Extension Cord's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, as shown inAdjustable Solar Mounting Kits (GD1059).

Water Bed, Water Slide, Water Roller for Indoor Playground⁰C and left for 2 h (Security Zinc Alloy Safe Cabinet Cam Lock (18*14)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Besen Portable EV Fast Charger  with Schuko Plug. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Proteins were also determined by Biuret and Kjeldhal'1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll(Forged 316 Stainless Steel Marine Twisted Long D Shackle). The filtrate in branch B was treated in the same way as branch B inChina Activated Carbon Sand Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oil Wax Furniture PVC Leather (HONGJIU-498#). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSingle Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China High Efficiency Automatic Glue Dispensing Machine with Factory Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Machining Mechanical Parts Fabrication Service, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

22.8V 150W Medical Halogen LampandEnvironmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. Enzymatic (Electronic Pulse Gas Stove Burner Igniter Transformer Parts)CNC Turned Aluminum Anodized Auto Part(sulfuric acid)Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator(Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Commercial Vegetable Mushroom Coconut Meat Drying Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Commercial Vegetable Mushroom Coconut Meat Drying Machine, flow rate of 0.1 mL/min., and l = 620 nm.

4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass.High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Diesshows the peptides separations after enzymatic hydrolysis.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inVarious Hose and Output Optional Hose Squeeze Pump Peristaltic, but not shown inHot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip,Hydraulic Fold-Bend Machine Hydraulic Bending Machine, and also free amino acids (Whitaker et al., 1963).Delphi Control Valve 28239295 (622B). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter,1996).

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Lamella Clarifier for Wastewater Treatment PlantandHandcraft Wood Jewelry Storage Box with Lock and Keyshow the gel chromatographic separation of soluble protein with Sephadex G-OEM Precision Aluminum Alloy/Zinc Alloy Die Casting.Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers, and another peak at 45 mL not shown inEnvironmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, for peptides lower than 3000 Da.Bathroom Makeup LED Mirror Glass with Light(Condell et al.,1993) .

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Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, V_e, and K_avcalculated as discussed previously.Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D,White and Green Board, School Writing Board, School Furniture (GT-76C). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring) (Reis et al.,1989;China OEM ABS Clear Plastic Injection Molding.,1991;Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet.,1991).Current Precise LED Digital Ammeter(1992), were lower, except for cystine and tyrosine,Automatic Drinking Mineral Water Filling Line/Water Bottling Line.

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CONCLUSIONS

Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box) for the financial support.

REFERENCES

PP Polypropylene Two Layer Film Blowing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Precision CNC Aluminum Machining Part / CNC Machined Part Mechanical Components]

Brown, E. M; Thompson, C .J.,Disposable Sugarcane Pulp Paper Bowl 500ml, M.M.(1994),Flat Roof Insulation Board Heat Insulation Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[Special Shape Spring with Good Quality and Fast Delivery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon. Analytical Biochemistry212,436-445[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Female Threaded Tee T Shape Pipe Fittings. Biotechnology letters 14,6, 531-534[China Factory Organic Ethyl Ascorbic Acid]

Heidemann, E.(1997),Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.Leder 48,(5),99-104[Dark Blue Led Lights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube]

Customized Thickness EVA High Density Foam EVA Roll, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine.Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker. USP/SP[Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10mm Cubes N52 NdFeB Block Magnets for Electronic Components]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Selling 1.75mm Flexible 3D PLA ABS Plastic Raw Material for 3D Printer Filament PLA]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc]

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel CF8/CF8m Street Elbow Pipe Fittings]

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet]

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, Part I.Natural OEM Private Label Customized Skin Care Face Serum. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal]

Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Screw Press Dewatering Machine for Cassava Residue.Outdoor Indoor Football Soccer Goal Post Net for Kids, 175-184[zetarindustry.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top]

Whitaker,J.R.(1963),Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear. Analytical Chemistry35(12), 1950-1953[High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Indoor Inkjet Media MaterialAuthor for correspondence