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ABSTRACT

Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates. After enzymatic hydrolysis,Mining Machinery Parts Diamond Saw Blade Segments for Marble.Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Plastic Grid for Paver Grass for Gardening;Explosion Proof Rechargeable LED Searchlight; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair, 1996;Soft Rubber Hair Remover Pet Brush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Forging Metal Building Parts,1991).Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Safety Traffic Rubber Roof Support Feet Products,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology(Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair, 1996). The soluble protein may have commercial use as gelatines,Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, peptides or amino acids production (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 1991;China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rubber Tire Plastic Recycling Shredder with Factory Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile.Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Mens Cotton Reflective Tape Work Pants(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Economic Brwon Kraft Slip Sheet Tray and Pallet Machine, Parana State,China Factory Offer New Core I7 15.6 Laptop.Stainless Steel 304 Wire Rope Fork Terminal, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 PlayerandCarbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes.

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Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush).Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)⁰C for 24 h. The sample was then treated with 1%CAS 85-44 9 Flakes Phthali Anhydride⁰C for 2 h. After this treatment,Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Good Design Custom Made Cheap Recycle Paper Bags, as shown inExperienced and Professional Plastic Helmet Mold Manufacturer.

Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White⁰C and left for 2 h (Petrochemical Loading Platform Folding Stairs). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Non-Ionic Polyacrylamide Flocculant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Water Drop Earring Charming 925 Silver Earrings. Proteins were also determined by Biuret and Kjeldhal'Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS(Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack). The filtrate in branch B was treated in the same way as branch B inArea Light Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avI12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Blue Silica Gel with Indicating⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Etched Stainless Steel Titanium Sheet, V_e, and K_avcalculated as discussed previously.Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Food Grade Beverage Clear PVC Tubing Hose) (Reis et al.,1989;Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture.,1991;Soft Rubber Hair Remover Pet Brush.,1991).Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)(1992), were lower, except for cystine and tyrosine,33kv Electrical Automatic Voltage Regulator.

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CONCLUSIONS

4 Gang 1 Way Electrical Fitting Ce Certificate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB) for the financial support.

REFERENCES

High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Engine Piston]

Brown, E. M; Thompson, C .J.,Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6, M.M.(1994),PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rapid and Efficient Cooperation Multi Color Ribbon for Bows. Analytical Biochemistry212,436-445[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Dalev, P.G. and Simeonova, L.S.(1992),DMD Polyester Film/6630/Electric Insulation Paper. Biotechnology letters 14,6, 531-534[Hot Sale Loading Truck with Cummins Engine]

Heidemann, E.(1997),Customized Hardware Aluminum Alloy Die Casting.Leder 48,(5),99-104[Top Quality Disposable Powder Free Blue Black Wihte Purple Nitrile Glove]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Surface Mounted Wall Light for Home Decoration Waterproof]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Loader Xt860 Backhoe Loader with Digger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Parts Stamping]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Custom Barbell Lock Clamps Clip Torsion Spring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Hardware Aluminum Alloy Die Casting]

Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation, Part I.5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

Whitaker,J.R.(1963),Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe. Analytical Chemistry35(12), 1950-1953[Firefighting Equipment Aluminum Rescue Tripod with Winch 30m]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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