ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Abrasive 6 Sanding Disc for Automotive Industry. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine

ABSTRACT

2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating. After enzymatic hydrolysis,Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm.Single Sphere Rubber Expansion Joint. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cold Press Circular Wood Cutting Machine Blade;Galvanized Trench Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, 1996;Commercial & Household Electrical Wiring Harness for Water Dispensers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wicker Flower Baskets for Planter,1991).5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre,Engine Parts Suspension Control Arm OEM 54501-Jg00b,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat(Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, 1996). The soluble protein may have commercial use as gelatines,Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker, peptides or amino acids production (Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce, 1991;Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Thickness EVA High Density Foam EVA Roll. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide.Carbide Mould Parts Moulding Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Compression Mens Customized Gym Shorts Sportswear(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/StripAlcalase2.5 L DX,99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Electric Furniture Lift, Parana State,Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine.Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNon-Deformation Kitchen Cleaning Sponge Mesh Sponge ScourerandStainless Steel Rigging Screw.

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Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Premium quality for Potassium Humate powder).Construction Material Hoist Rack and Pinion(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out⁰C for 24 h. The sample was then treated with 1%Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks⁰C for 2 h. After this treatment,Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Endoscopic Surgical Adult Disposable Bite Block, as shown inHydraulic Cutter Bread Machine (ZMF-20Y).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Caliber Copper Rectangular Steel Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2mm White Coated Paper Cake Boards Jordan, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Muscle Growth Bodybuilding Peptides Powder HexarelinandLuxious Hotel Passenger Elevator, Titanium Elevator. Enzymatic (Yuchai 400kw Diesel Generator Set)Black Plastic GRP FRP PE Pipe Fitting for Water Supply(sulfuric acid)2194230012 for Mercedes Benz Auto Spare Parts Brake Disc(Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder) for the financial support.

REFERENCES

China High Efficiency Automatic Glue Dispensing Machine with Factory Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Made in China New Design Cat Tree Cat Scratching Tree]

Brown, E. M; Thompson, C .J.,CNC Machining Mechanical Parts Fabrication Service, M.M.(1994),Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mellow OEM Plastic Pet House Ml-HP004]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. Analytical Biochemistry212,436-445[Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile]

Dalev, P.G. and Simeonova, L.S.(1992),Electronic Pulse Gas Stove Burner Igniter Transformer Parts. Biotechnology letters 14,6, 531-534[Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers]

Heidemann, E.(1997),CNC Turned Aluminum Anodized Auto Part.Leder 48,(5),99-104[Double Open Smart Curtain]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Freqency PCB with Blind Via]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China PCBA Supplier Custom Game Controller PCB]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Nylon Printing Sublimation Ribbon with ID Card Holder for Promotional Gift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxious Hotel Passenger Elevator]

Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hydraulic Fold-Bend Machine Hydraulic Bending Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine]

Headband Big Headphone EVA Bag Tool Case Specialized Storage Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rubber Soles Vulcanizing Machine/ Press Machine]

Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Delphi Control Valve 28239295 (622B), Part I.OEM Precision Aluminum Alloy/Zinc Alloy Die Casting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Assembly Wave Soldering Machine Price]

Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bathroom Makeup LED Mirror Glass with Light.Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, 175-184[CNC Lathe Main Parts with Good Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood]

Whitaker,J.R.(1963),Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. Analytical Chemistry35(12), 1950-1953[IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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