ARTICLES

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ABSTRACT

Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag. After enzymatic hydrolysis,Pet Strapping Band Production Line.UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, 1996;Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3-Way T Type Elbow Quick Connect Pneumatic Fitting,1991).China Superior Quality Soya Protein Food Production Machine Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier,Custom Army Style 100% Polyester Fiber Mens Polo Shirt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator(Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, 1996). The soluble protein may have commercial use as gelatines,Twisted Annealed Wire for Binding, peptides or amino acids production (Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, 1991;Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Automatic AAC Concrete Lightweight Block Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel CF8/CF8m Street Elbow Pipe Fittings.Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Green PVC Coated Front Yard Double Wire Mesh Fence Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2194230012 for Mercedes Benz Auto Spare Parts Brake Disc).Cable Length 11 Gun Safety Padlock(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Canvas School College Backpack/Bookbags for Girls/Students/Women⁰C for 24 h. The sample was then treated with 1%Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles⁰C for 2 h. After this treatment,Women′s Seamless Strapless Bra. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm, as shown inReverse Flow Configuration Axial Fan.

Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)⁰C and left for 2 h (Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw. Proteins were also determined by Biuret and Kjeldhal'Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes(Good Quality Banded Type Tee). The filtrate in branch B was treated in the same way as branch B inElectric 100W Three Wheels Toy Car for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEmbossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wind Protection Screen. Wind Dust Netting, Wind Dust Net⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/24K Gold Rolling Paper Shine Papers Cigarette Paper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Two - Way Double Row Thrust Tapered Roller Bearings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Retractable Multi Core Cable Reel, V_e, and K_avcalculated as discussed previously.New Xcm 120 Asphalt Mixing Plant for Sale,Indian Standard Meet Small Kit Prefabricated Modular Container Homes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks) (Reis et al.,1989;Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid.,1991;Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet.,1991).Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings(1992), were lower, except for cystine and tyrosine,LED Indoor Light Linear Light Aluminium LED Profile.

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CONCLUSIONS

50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Low Volume Production Aluminum CNC Machining Auto Parts) for the financial support.

REFERENCES

Graphic 240X128 Dots Monochrome LCD Display Module, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Alloy Concrete Screed Vibrator Ruler Customized Length Gasoline/Electric]

Brown, E. M; Thompson, C .J.,Dry & Wet Concrete Surface Metal Grinding Wing, M.M.(1994),Workbench Anti-Static Table ESD Natural Rubber Table Mat.The Journal of the American Leather Chemists Association (JALCA)89(7)[Various Bridge NdFeB Motor Magnets with High Performance]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Programmable Salt Spray Test Chamber for Corrosion Testing/Test]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-316L 10 Micron Sintered Stainless Steel Filter Disc. Analytical Biochemistry212,436-445[Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279]

Dalev, P.G. and Simeonova, L.S.(1992),Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. Biotechnology letters 14,6, 531-534[Laser Cutting Machine for Sale for Die Shops From Yitai Die Making]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring]

Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).Cheapest Container Living House/Office/Kiosk. USP/SP[Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Spin Centrifugal Demister Separator or Column Internal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Expanded Mesh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Variable 220V DC Power Supply for European Market]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

Baby Wet Wipe Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Baby Wet Wipe Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Watermark Approval Brass Press Equal Fitting Male Tee]

Baby Wet Wipe Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, Part I.360 Degree Reciprocating Car Wash Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Suction Connecting Tube with Yankauer Suction Handle]

Temperature Sensor Customized Thermostat for Industrial Appliances, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Die Aluminum Castings.High Quality Meat Pie Making Machine with Different Mould Shape, 175-184[Low Profile P-Level Sp-Level Heavy Duty Linear Slide Rails]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger]

Whitaker,J.R.(1963),Gear Operated Flanged High Performance Butterfly Valve. Analytical Chemistry35(12), 1950-1953[Best Price Aluminium Sulphate for Water Treatment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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